Curated Information
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Home > Curated Information Page > PubMed Id: 9218810
Jefferies HB, et al. (1997) Rapamycin suppresses 5'TOP mRNA translation through inhibition of p70s6k. EMBO J 16, 3693-704 9218810
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T252-p - p70S6K (human)
Modsite: HDGtVtHtFCGtIEY SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Effect of modification (process):  translation, altered
Comments:  affects phosphorylation of T412, S427, and S434 of p70S6K

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  translation, altered

S427-p - p70S6K (human)
Modsite: EsVKEkFsFEPKIRs SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S427‑p, p70S6K iso2 (human): S404‑p, p70S6K (mouse): S427‑p, p70S6K (rat): S427‑p, p70S6K iso2 (rat): S404‑p, p70S6K (fruit fly): R413‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S434-p - p70S6K (human)
Modsite: sFEPKIRsPRRFIGs SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S434‑p, p70S6K iso2 (human): S411‑p, p70S6K (mouse): S434‑p, p70S6K (rat): S434‑p, p70S6K iso2 (rat): S411‑p, p70S6K (fruit fly): S418‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S441-p - p70S6K (human)
Modsite: sPRRFIGsPRtPVsP SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S441‑p, p70S6K iso2 (human): S418‑p, p70S6K (mouse): S441‑p, p70S6K (rat): S441‑p, p70S6K iso2 (rat): S418‑p, p70S6K (fruit fly): Q425‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

T444-p - p70S6K (human)
Modsite: RFIGsPRtPVsPVkF SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): S429‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S447-p - p70S6K (human)
Modsite: GsPRtPVsPVkFsPG SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): S430‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse