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Home > Curated Information Page > PubMed Id: 23230143
Nadeem L, et al. (2013) Cytoplasmic mislocalization of p27 and CDK2 mediates the anti-migratory and anti-proliferative effects of Nodal in human trophoblast cells. J Cell Sci 126, 445-53 23230143
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo immunoassay, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal increase
Downstream Regulation
Effect of modification (function):  intracellular localization

T157-p - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal decrease

T187-p - p27Kip1 (human)
Modsite: NAGsVEQtPKKPGLR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal decrease

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal decrease

S25-p - STMN1 (human)
Modsite: QAFELILsPrskEsV SwissProt Entrez-Gene
Orthologous residues
STMN1 (human): S25‑p, STMN1 iso2 (human): S25‑p, STMN1 (mouse): S25‑p, STMN1 (rat): S25‑p, STMN1 (chicken): G25‑p, STMN1 (cow): S25‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal increase
Cdk2/5_inhibitor Nodal inhibit treatment-induced increase

S38-p - STMN1 (human)
Modsite: sVPEFPLsPPkKkDL SwissProt Entrez-Gene
Orthologous residues
STMN1 (human): S38‑p, STMN1 iso2 (human): S38‑p, STMN1 (mouse): S38‑p, STMN1 (rat): S38‑p, STMN1 (chicken): S38‑p, STMN1 (cow): S38‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HTR8/SVneo (trophoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Nodal increase
Cdk2/5_inhibitor Nodal inhibit treatment-induced increase