Wang Y, et al. (2013) Phosphorylation and Recruitment of BAF60c in Chromatin Remodeling for Lipogenesis in Response to Insulin. Mol Cell 49, 283-97 23219531

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S247-p - SMARCD3 (human) ▲

Modsite: VKRPGDLsVRCTLLL SwissProt Entrez-Gene Orthologous residues SMARCD3 (human): S247‑p, SMARCD3 (mouse): S247‑p, SMARCD3 (rat): S234‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  293 (epithelial), HepG2 (hepatic), liver Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCZ (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) transfection of dominant-negative enzyme, transfection of wild-type enzyme, competition with site-specific peptide, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes meal feeding increase insulin increase siRNA decrease PKCZ-siRNA decreases phosphorylation Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  chromatin organization, altered, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays USF1 (human) Induces pull-down assay, co-immunoprecipitation

K235-ac - USF1 (human) ▲

Modsite: DCSMESTkSGQSkGG SwissProt Entrez-Gene Orthologous residues USF1 (human): K235‑ac, USF1 (mouse): K235‑ac, USF1 (rat): K235‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  293 (epithelial), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes meal feeding increase insulin increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SMARCD3 (human) Induces co-immunoprecipitation Comments:  K235 acetylation induces recruitment of SMARCD3 to the promoters of lipogenic genes

S262-p - USF1 (human) ▲

Modsite: RQSNHRLsEELQGLD SwissProt Entrez-Gene Orthologous residues USF1 (human): S262‑p, USF1 (mouse): S262‑p, USF1 (rat): S262‑p Characterization Methods used to characterize site in vivo:  modification-specific antibody, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE DNAPK (human) genetic knockout/knockin of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes meal feeding increase insulin increase Downstream Regulation Effect of modification (function):  acetylation, molecular association, regulation Comments:  increases acetylation at USF1-K235