Curated Information
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Home > Curated Information Page > PubMed Id: 22487004
Smillie KJ, Cousin MA (2012) Akt/PKB controls the activity-dependent bulk endocytosis of synaptic vesicles. Traffic 13, 1004-11 22487004
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
action_potential increase increasing frequency
Akt-I-1,2 action_potential inhibit treatment-induced increase
Akt_inhibitor_X action_potential inhibit treatment-induced increase
APV, CNQX action_potential no effect upon treatment-induced increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
action_potential increase increasing frequency
Akt-I-1,2 action_potential inhibit treatment-induced increase
Akt_inhibitor_X action_potential inhibit treatment-induced increase
APV, CNQX action_potential no effect upon treatment-induced increase

S774-p - DYN1 (rat)
Modsite: SVPAGRRsPTSsPTP SwissProt Entrez-Gene
Orthologous residues
DYN1 (human): S774‑p, DYN1 iso2 (human): S774‑p, DYN1 (mouse): S774‑p, DYN1 iso3 (mouse): S774‑p, DYN1 iso4 (mouse): S774‑p, DYN1 iso6 (mouse): S770‑p, DYN1 (rat): S774‑p, DYN1 iso2 (rat): S774‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
action_potential decrease increasing frequency
Akt-I-1,2 action_potential inhibit treatment-induced decrease
Akt_inhibitor_X action_potential inhibit treatment-induced decrease
APV, CNQX action_potential no effect upon treatment-induced increase

S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
action_potential increase increasing frequency
Akt-I-1,2 action_potential inhibit treatment-induced increase
Akt_inhibitor_X action_potential inhibit treatment-induced increase
APV, CNQX action_potential no effect upon treatment-induced increase

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
action_potential increase increasing frequency
Akt-I-1,2 action_potential inhibit treatment-induced increase
Akt_inhibitor_X action_potential inhibit treatment-induced increase
APV, CNQX action_potential no effect upon treatment-induced increase