Curated Information
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Home > Curated Information Page > PubMed Id: 22981863
Rouquette-Jazdanian AK, et al. (2012) LAT-Independent Erk Activation via Bam32-PLC-γ1-Pak1 Complexes: GTPase-Independent Pak1 Activation. Mol Cell 48, 298-312 22981863
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y139-p - DAPP1 (human)
Modsite: kVEEPsIyEsVRVHT SwissProt Entrez-Gene
Orthologous residues
DAPP1 (human): Y139‑p, DAPP1 iso2 (human): Y139‑p, DAPP1 (mouse): Y139‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  CD4+ (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (human) co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A CD3E (human) increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  pS141 negatively regulates pY139 and vice versa

S141-p - DAPP1 (human)
Modsite: EEPsIyEsVRVHTAM SwissProt Entrez-Gene
Orthologous residues
DAPP1 (human): S141‑p, DAPP1 iso2 (human): S141‑p, DAPP1 (mouse): S141‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  CD4+ (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A CD3E (human) increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation
Effect of modification (process):  signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces co-immunoprecipitation
Comments:  pS141 negatively regulates pY139 and vice versa

S199-p - PAK1 (human)
Modsite: PRPEHtKsVyTRsVI SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S199‑p, PAK1 (mouse): S199‑p, PAK1 (rat): S198‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  CD4+ (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PAK1 (human) co-immunoprecipitation
Comments:  Autophosphorylation

S204-p - PAK1 (human)
Modsite: tKsVyTRsVIEPLPV SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S204‑p, PAK1 (mouse): S204‑p, PAK1 (rat): S203‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  CD4+ (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PAK1 (human) co-immunoprecipitation
Comments:  Autophosphorylation