Curated Information
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Home > Curated Information Page > PubMed Id: 17289681
Lunn JA, Jacamo R, Rozengurt E (2007) Preferential phosphorylation of focal adhesion kinase tyrosine 861 is critical for mediating an anti-apoptotic response to hyperosmotic stress. J Biol Chem 282, 10370-9 17289681
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y397-p - FAK (mouse)
Modsite: sVsEtDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IEC-18 (epithelial), SYF (fibroblast) [Src (mouse)]
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
sucrose Src (mouse) increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
LPA increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
sucrose increase
latrunculin_A sucrose no effect upon treatment-induced increase
cytochalasin_D, sucrose sucrose no effect upon treatment-induced increase
angiotensin_2 no change compared to control
angiotensin_2, latrunculin_A no change compared to control
angiotensin_2, cytochalasin_D no change compared to control
latrunculin_A no change compared to control
cytochalasin_D increase
sorbitol increase
latrunculin_A sorbitol no change compared to control
cytochalasin_D sorbitol no change compared to control
latrunculin_A no change compared to control
cytochalasin_D decrease

Y576-p - FAK (mouse)
Modsite: RyMEDstyyKASKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IEC-18 (epithelial), SYF (fibroblast) [Src (mouse)]
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
sucrose Src (mouse) increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
LPA increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
sucrose increase
latrunculin_A sucrose no effect upon treatment-induced increase
cytochalasin_D sucrose inhibit treatment-induced increase
angiotensin_2 increase
latrunculin_A angiotensin_2 inhibit treatment-induced increase
cytochalasin_D angiotensin_2 inhibit treatment-induced increase
latrunculin_A decrease
cytochalasin_D decrease
sorbitol decrease
latrunculin_A sorbitol increase
cytochalasin_D sorbitol no change compared to control
latrunculin_A no change compared to control
cytochalasin_D decrease

Y861-p - FAK (mouse)
Modsite: PTGNQHIyQPVGKPD SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y861‑p, FAK iso2 (human): Y688‑p, FAK iso5 (human): Y861‑p, FAK (mouse): Y861‑p, FAK iso2 (mouse): Y892‑p, FAK iso4 (mouse): Y861‑p, FAK iso9 (mouse): Y169‑p, FAK (rat): Y861‑p, FAK (chicken): Y863‑p, FAK iso5 (chicken): Y169‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IEC-18 (epithelial), SYF (fibroblast) [Src (mouse)]
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sucrose increase
angiotensin_2 increase
urea no change compared to control
sorbitol increase
sucrose increase
SU6656 sucrose inhibit treatment-induced increase
PP2 sucrose inhibit treatment-induced increase
sucrose Src (mouse) increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
LPA increase in cells lacking Yes and Fyn; no increase in cells lacking Src, Yes, and Fyn
sucrose increase
latrunculin_A sucrose no effect upon treatment-induced increase
cytochalasin_D sucrose no effect upon treatment-induced increase
angiotensin_2 no change compared to control
angiotensin_2, latrunculin_A no change compared to control
angiotensin_2, cytochalasin_D no change compared to control
latrunculin_A no change compared to control
cytochalasin_D no change compared to control
sorbitol increase
latrunculin_A sorbitol augment treatment-induced increase
cytochalasin_D sorbitol augment treatment-induced increase
latrunculin_A no change compared to control
cytochalasin_D decrease
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  cytoskeletal reorganization
Comments:  Y861 phosphorylation is necessary for cell survival after osmotic stress.