Curated Information
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Home > Curated Information Page > PubMed Id: 12972425
Goldberg GS, et al. (2003) Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells. J Biol Chem 278, 46533-40 12972425
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y226-p - P130Cas (rat)
Modsite: SKTQQGLyQAPGPNP SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y128‑p, P130Cas iso2 (human): Y146‑p, P130Cas iso6 (human): Y174‑p, P130Cas iso7 (human): Y146‑p, P130Cas iso8 (human): Y128‑p, P130Cas (mouse): Y132‑p, P130Cas (rat): Y226‑p, P130Cas iso2 (rat): Y132‑p

Y263-p - P130Cas (rat)
Modsite: PSPATDLyQVPPGPG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y165‑p, P130Cas iso2 (human): Y183‑p, P130Cas iso6 (human): Y211‑p, P130Cas iso7 (human): Y183‑p, P130Cas iso8 (human): Y165‑p, P130Cas (mouse): Y169‑p, P130Cas (rat): Y263‑p, P130Cas iso2 (rat): Y169‑p

Y277-p - P130Cas (rat)
Modsite: GSPAQDIyQVPPSAG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y179‑p, P130Cas iso2 (human): Y197‑p, P130Cas iso6 (human): Y225‑p, P130Cas iso7 (human): Y197‑p, P130Cas iso8 (human): Y179‑p, P130Cas (mouse): Y183‑p, P130Cas (rat): Y277‑p, P130Cas iso2 (rat): Y183‑p

Y290-p - P130Cas (rat)
Modsite: AGTGHDIyQVPPSLD SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y192‑p, P130Cas iso2 (human): Y210‑p, P130Cas iso6 (human): Y238‑p, P130Cas iso7 (human): Y210‑p, P130Cas iso8 (human): Y192‑p, P130Cas (mouse): Y196‑p, P130Cas (rat): Y290‑p, P130Cas iso2 (rat): Y196‑p

Y332-p - P130Cas (rat)
Modsite: SQAEQDEyDTPRHLL SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y234‑p, P130Cas iso2 (human): Y252‑p, P130Cas iso6 (human): Y280‑p, P130Cas iso7 (human): Y252‑p, P130Cas iso8 (human): Y234‑p, P130Cas (mouse): Y238‑p, P130Cas (rat): Y332‑p, P130Cas iso2 (rat): Y238‑p

Y347-p - P130Cas (rat)
Modsite: APGSQDIyDVPPVRG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y249‑p, P130Cas iso2 (human): Y267‑p, P130Cas iso6 (human): Y295‑p, P130Cas iso7 (human): Y267‑p, P130Cas iso8 (human): Y249‑p, P130Cas (mouse): Y253‑p, P130Cas (rat): Y347‑p, P130Cas iso2 (rat): Y253‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (chicken)

Y365-p - P130Cas (rat)
Modsite: NQYGQEVyDTPPMAV SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y267‑p, P130Cas iso2 (human): Y285‑p, P130Cas iso6 (human): Y313‑p, P130Cas iso7 (human): Y285‑p, P130Cas iso8 (human): Y267‑p, P130Cas (mouse): Y271‑p, P130Cas (rat): Y365‑p, P130Cas iso2 (rat): Y271‑p

Y385-p - P130Cas (rat)
Modsite: RDPLLDVyDVPPSVE SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y287‑p, P130Cas iso2 (human): Y305‑p, P130Cas iso6 (human): Y333‑p, P130Cas iso7 (human): Y305‑p, P130Cas iso8 (human): Y287‑p, P130Cas (mouse): Y291‑p, P130Cas (rat): Y385‑p, P130Cas iso2 (rat): Y291‑p

Y404-p - P130Cas (rat)
Modsite: PSNHHSVyDVPPSVS SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y306‑p, P130Cas iso2 (human): Y324‑p, P130Cas iso6 (human): Y352‑p, P130Cas iso7 (human): Y324‑p, P130Cas iso8 (human): Y306‑p, P130Cas (mouse): Y310‑p, P130Cas (rat): Y404‑p, P130Cas iso2 (rat): Y310‑p

Y485-p - P130Cas (rat)
Modsite: RPGPGTLyDVPRERV SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y387‑p, P130Cas iso2 (human): Y405‑p, P130Cas iso6 (human): Y433‑p, P130Cas iso7 (human): Y405‑p, P130Cas iso8 (human): Y387‑p, P130Cas (mouse): Y391‑p, P130Cas (rat): Y485‑p, P130Cas iso2 (rat): Y391‑p

Y508-p - P130Cas (rat)
Modsite: SVIDDGVyAVPPPAE SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y410‑p, P130Cas iso2 (human): Y428‑p, P130Cas iso6 (human): Y456‑p, P130Cas iso7 (human): Y428‑p, P130Cas iso8 (human): Y410‑p, P130Cas (mouse): Y414‑p, P130Cas (rat): Y508‑p, P130Cas iso2 (rat): Y414‑p

Y132-p - P130Cas iso2 (rat)
Modsite: SKTQQGLyQAPGPNP SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y128‑p, P130Cas iso2 (human): Y146‑p, P130Cas iso6 (human): Y174‑p, P130Cas iso7 (human): Y146‑p, P130Cas iso8 (human): Y128‑p, P130Cas (mouse): Y132‑p, P130Cas (rat): Y226‑p, P130Cas iso2 (rat): Y132‑p

Y169-p - P130Cas iso2 (rat)
Modsite: PSPATDLyQVPPGPG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y165‑p, P130Cas iso2 (human): Y183‑p, P130Cas iso6 (human): Y211‑p, P130Cas iso7 (human): Y183‑p, P130Cas iso8 (human): Y165‑p, P130Cas (mouse): Y169‑p, P130Cas (rat): Y263‑p, P130Cas iso2 (rat): Y169‑p

Y183-p - P130Cas iso2 (rat)
Modsite: GSPAQDIyQVPPSAG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y179‑p, P130Cas iso2 (human): Y197‑p, P130Cas iso6 (human): Y225‑p, P130Cas iso7 (human): Y197‑p, P130Cas iso8 (human): Y179‑p, P130Cas (mouse): Y183‑p, P130Cas (rat): Y277‑p, P130Cas iso2 (rat): Y183‑p

Y196-p - P130Cas iso2 (rat)
Modsite: AGTGHDIyQVPPSLD SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y192‑p, P130Cas iso2 (human): Y210‑p, P130Cas iso6 (human): Y238‑p, P130Cas iso7 (human): Y210‑p, P130Cas iso8 (human): Y192‑p, P130Cas (mouse): Y196‑p, P130Cas (rat): Y290‑p, P130Cas iso2 (rat): Y196‑p

Y238-p - P130Cas iso2 (rat)
Modsite: SQAEQDEyDTPRHLL SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y234‑p, P130Cas iso2 (human): Y252‑p, P130Cas iso6 (human): Y280‑p, P130Cas iso7 (human): Y252‑p, P130Cas iso8 (human): Y234‑p, P130Cas (mouse): Y238‑p, P130Cas (rat): Y332‑p, P130Cas iso2 (rat): Y238‑p

Y253-p - P130Cas iso2 (rat)
Modsite: APGSQDIyDVPPVRG SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y249‑p, P130Cas iso2 (human): Y267‑p, P130Cas iso6 (human): Y295‑p, P130Cas iso7 (human): Y267‑p, P130Cas iso8 (human): Y249‑p, P130Cas (mouse): Y253‑p, P130Cas (rat): Y347‑p, P130Cas iso2 (rat): Y253‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (chicken)

Y271-p - P130Cas iso2 (rat)
Modsite: NQYGQEVyDTPPMAV SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y267‑p, P130Cas iso2 (human): Y285‑p, P130Cas iso6 (human): Y313‑p, P130Cas iso7 (human): Y285‑p, P130Cas iso8 (human): Y267‑p, P130Cas (mouse): Y271‑p, P130Cas (rat): Y365‑p, P130Cas iso2 (rat): Y271‑p

Y291-p - P130Cas iso2 (rat)
Modsite: RDPLLDVyDVPPSVE SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y287‑p, P130Cas iso2 (human): Y305‑p, P130Cas iso6 (human): Y333‑p, P130Cas iso7 (human): Y305‑p, P130Cas iso8 (human): Y287‑p, P130Cas (mouse): Y291‑p, P130Cas (rat): Y385‑p, P130Cas iso2 (rat): Y291‑p

Y310-p - P130Cas iso2 (rat)
Modsite: PSNHHSVyDVPPSVS SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y306‑p, P130Cas iso2 (human): Y324‑p, P130Cas iso6 (human): Y352‑p, P130Cas iso7 (human): Y324‑p, P130Cas iso8 (human): Y306‑p, P130Cas (mouse): Y310‑p, P130Cas (rat): Y404‑p, P130Cas iso2 (rat): Y310‑p

Y391-p - P130Cas iso2 (rat)
Modsite: RPGPGTLyDVPRERV SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y387‑p, P130Cas iso2 (human): Y405‑p, P130Cas iso6 (human): Y433‑p, P130Cas iso7 (human): Y405‑p, P130Cas iso8 (human): Y387‑p, P130Cas (mouse): Y391‑p, P130Cas (rat): Y485‑p, P130Cas iso2 (rat): Y391‑p

Y414-p - P130Cas iso2 (rat)
Modsite: SVIDDGVyAVPPPAE SwissProt Entrez-Gene
Orthologous residues
P130Cas (human): Y410‑p, P130Cas iso2 (human): Y428‑p, P130Cas iso6 (human): Y456‑p, P130Cas iso7 (human): Y428‑p, P130Cas iso8 (human): Y410‑p, P130Cas (mouse): Y414‑p, P130Cas (rat): Y508‑p, P130Cas iso2 (rat): Y414‑p