Curated Information
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Home > Curated Information Page > PubMed Id: 17237771
Liang J, et al. (2007) The energy sensing LKB1-AMPK pathway regulates p27(kip1) phosphorylation mediating the decision to enter autophagy or apoptosis. Nat Cell Biol 9, 218-24 17237771
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S80-p - ACC1 (human)
Modsite: LHIRssMsGLHLVkQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting
Disease tissue studied:  bone cancer, breast cancer, melanoma skin cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell), U2OS (bone cell), WM239A (melanocyte), WM35 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

T183-p - AMPKA1 (human)
Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting
Disease tissue studied:  bone cancer, breast cancer, melanoma skin cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell), U2OS (bone cell), WM239A (melanocyte), WM35 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation increase
siRNA glucose_starvation p27Kip1 (human) no effect upon treatment-induced increase
LY294002 increase

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting
Disease tissue studied:  bone cancer, breast cancer, melanoma skin cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell), U2OS (bone cell), WM239A (melanocyte), WM35 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MG132 increase
glucose_starvation increase
glucose_starvation LKB1 (human) inhibit treatment-induced increase null
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Effect of modification (process):  cell cycle regulation, cell growth, altered

S79-p - ACC1 (mouse)
Modsite: FHMRSsMsGLHLVKQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase
glucose_starvation increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation no change compared to control

T183-p - AMPKA1 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase
glucose_starvation increase

T197-p - p27Kip1 (mouse)
Modsite: KPGLRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase
glucose_starvation increase
siRNA glucose_starvation LKB1 (mouse) inhibit treatment-induced increase