Sahin B, et al. (2007) Evaluation of neuronal phosphoproteins as effectors of caffeine and mediators of striatal adenosine A2A receptor signaling. Brain Res 1129, 1-14 17157277

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T286-p - CAMK2A (mouse) ▲

Modsite: sCMHRQEtVDCLkkF SwissProt Entrez-Gene Orthologous residues CAMK2A (human): T286‑p, CAMK2A iso2 (human): T286‑p, CAMK2A (mouse): T286‑p, CAMK2A iso2 (mouse): ‑, CAMK2A (rat): T286‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin no change compared to control

S119-p - CREB (mouse) ▲

Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene Orthologous residues CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S133‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 increase caffeine no change compared to control

T34-p - DARPP-32 (mouse) ▲

Modsite: MIRRRRPtPAMLFRV SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): T34‑p, DARPP‑32 iso2 (human): ‑, DARPP‑32 (mouse): T34‑p, DARPP‑32 (rat): T34‑p, DARPP‑32 (cow): T34‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 increase caffeine decrease

T75-p - DARPP-32 (mouse) ▲

Modsite: RPNPCAYtPPSLKAV SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): T75‑p, DARPP‑32 iso2 (human): T39‑p, DARPP‑32 (mouse): T75‑p, DARPP‑32 (rat): T75‑p, DARPP‑32 (cow): T75‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes caffeine increase

S130-p - DARPP-32 (mouse) ▲

Modsite: EEDEEEDsQAEVLKG SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): S137‑p, DARPP‑32 iso2 (human): S101‑p, DARPP‑32 (mouse): S130‑p, DARPP‑32 (rat): S137‑p, DARPP‑32 (cow): S135‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes caffeine no change compared to control

T203-p - ERK1 (mouse) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes caffeine no change compared to control colforsin increase CGS_21680 increase

Y205-p - ERK1 (mouse) ▲

Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene Orthologous residues ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes caffeine no change compared to control colforsin increase CGS_21680 increase

T183-p - ERK2 (mouse) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CGS_21680 increase caffeine no change compared to control colforsin increase

Y185-p - ERK2 (mouse) ▲

Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CGS_21680 increase caffeine no change compared to control colforsin increase

S863-p - GluR1 (mouse) ▲

Modsite: tstLPRNsGAGASGG SwissProt Entrez-Gene Orthologous residues GluR1 (human): S863‑p, GluR1 iso6 (human): S873‑p, GluR1 (mouse): S863‑p, GluR1 (rat): S863‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 increase caffeine no change compared to control

S897-p - NMDAR1 (mouse) ▲

Modsite: sFKRRRssKDTSTGG SwissProt Entrez-Gene Orthologous residues NMDAR1 (human): S897‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S918‑p, NMDAR1 (mouse): S897‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S918‑p, NMDAR1 (rat): S897‑p, NMDAR1 iso7 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 increase caffeine no change compared to control

S94-p - Spinophilin (mouse) ▲

Modsite: SDRGVRLsLPRAssL SwissProt Entrez-Gene Orthologous residues Spinophilin (human): S94‑p, Spinophilin (mouse): S94‑p, Spinophilin (rat): S94‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 increase caffeine decrease

S9-p - SYN1 (mouse) ▲

Modsite: NYLRRRLsDsNFMAN SwissProt Entrez-Gene Orthologous residues SYN1 (human): S9‑p, SYN1 iso2 (human): S9‑p, SYN1 (mouse): S9‑p, SYN1 iso1 (mouse): S9‑p, SYN1 iso3 (mouse): S9‑p, SYN1 (rat): S9‑p, SYN1 (cow): S9‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 no change compared to control

S40-p - TH (mouse) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CGS_21680 no change compared to control caffeine no change compared to control