Curated Information
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Home > Curated Information Page > PubMed Id: 17130121
Yang W, et al. (2007) Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem 282, 3799-808 17130121
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
myostatin ACVR2B (mouse) decrease ACVR2B siRNA inhibits decrease
IGF-1 myostatin ACVR2B (mouse) inhibit treatment-induced decrease
wortmannin decrease
IGF-1 IGF1R (mouse) increase IGF1R siRNA inhibits increase

T286-p - CCND1 (mouse)
Modsite: EEAGLACtPtDVRDV SwissProt Entrez-Gene
Orthologous residues
CCND1 (human): T286‑p, CCND1 (mouse): T286‑p, CCND1 (rat): T286‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
myostatin increase
Downstream Regulation
Effect of modification (function):  protein degradation

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
myostatin ACVR2B (mouse) decrease ACVR2B siRNA inhibits decrease
IGF-1 myostatin ACVR2B (mouse) inhibit treatment-induced decrease
wortmannin decrease