Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 7642584
Giannini E, Brouchon L, Boulay F (1995) Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo. J Biol Chem 270, 19166-72 7642584
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S314-p - C5aR (human)
Modsite: FQGRLRKsLPsLLRN SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S314‑p, C5aR (mouse): S315‑p, C5aR iso3 (mouse): S315‑p, C5aR (rat): S316‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase

S317-p - C5aR (human)
Modsite: RLRKsLPsLLRNVLt SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S317‑p, C5aR (mouse): S318‑p, C5aR iso3 (mouse): S318‑p, C5aR (rat): S319‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase

S327-p - C5aR (human)
Modsite: RNVLtEEsVVREsKs SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S327‑p, C5aR (mouse): S328‑p, C5aR iso3 (mouse): S328‑p, C5aR (rat): S329‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase

S332-p - C5aR (human)
Modsite: EEsVVREsKsFTRsT SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S332‑p, C5aR (mouse): S333‑p, C5aR iso3 (mouse): S333‑p, C5aR (rat): S334‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase

S334-p - C5aR (human)
Modsite: sVVREsKsFTRsTVD SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S334‑p, C5aR (mouse): T335‑p, C5aR iso3 (mouse): T335‑p, C5aR (rat): S336‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase

S338-p - C5aR (human)
Modsite: EsKsFTRsTVDTMAQ SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S338‑p, C5aR (mouse): S339‑p, C5aR iso3 (mouse): S339‑p, C5aR (rat): S340‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
C5a increase