Curated Information
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Home > Curated Information Page > PubMed Id: 16651424
Mungamuri SK, Yang X, Thor AD, Somasundaram K (2006) Survival signaling by Notch1: mammalian target of rapamycin (mTOR)-dependent inhibition of p53. Cancer Res 66, 4715-24 16651424
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S65-p - 4E-BP1 (human)
Modsite: FLMECrNsPVtktPP SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p, 4E‑BP1 (cow): S65‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) increase
Notch 1 (human) no change compared to control
p53 (human) decrease
Notch 1 (human) increase
rapamycin Notch 1 (human) Notch 1 (human) inhibit treatment-induced increase

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) increase
Notch 1 (human) increase
Notch 1 (human) no change compared to control

S256-p - FOXO1A (human)
Modsite: sPrRrAAsMDNNskF SwissProt Entrez-Gene
Orthologous residues
FOXO1A (human): S256‑p, FOXO1A (mouse): S253‑p, FOXO1A (rat): S250‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) decrease
Notch 1 (human) no change compared to control

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) increase
Notch 1 (human) no change compared to control
p53 (human) decrease

S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) increase
Notch 1 (human) no change compared to control
p53 (human) decrease

S2481-p - mTOR (human)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
p53 (human), Notch 1 (human) increase
Notch 1 (human) no change compared to control
p53 (human) decrease

S15-p - p53 (human)
Modsite: PsVEPPLsQEtFsDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H460 (pulmonary), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  apoptosis, induced
Comments:  Notch-1 inhibited p53 activating phosphorylation, and its nuclear localization.

S20-p - p53 (human)
Modsite: PLsQEtFsDLWkLLP SwissProt Entrez-Gene
Orthologous residues
p53 (human): S20‑p, p53 iso2 (human): S20‑p, p53 iso4 (human): , p53 (mouse): S23‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (green monkey): S20‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H460 (pulmonary), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  apoptosis, induced
Comments:  Notch-1 inhibited p53 activating phosphorylation, and its nuclear localization.

S392-p - p53 (human)
Modsite: FktEGPDsD______ SwissProt Entrez-Gene
Orthologous residues
p53 (human): S392‑p, p53 iso2 (human): , p53 iso4 (human): S353‑p, p53 (mouse): S389‑p, p53 iso2 (mouse): , p53 (rat): S390‑p, p53 (rabbit): S390‑p, p53 (green monkey): S392‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H460 (pulmonary), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  apoptosis, induced
Comments:  Notch-1 inhibited p53 activating phosphorylation, and its nuclear localization.

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Notch 1 (human) increase
rapamycin Notch 1 (human) Notch 1 (human) inhibit treatment-induced increase