Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22412986
Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC (2012) Activation of interferon regulatory factor 5 by site specific phosphorylation. PLoS One 7, e33098 22412986
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T10-p - IRF5 (human)
Modsite: QSIPVAPtPPRRVRL SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): T10‑p, IRF5 iso2 (human): T10‑p, IRF5 (mouse): P10‑p, IRF5 (rat): T10‑p

S158-p - IRF5 (human)
Modsite: QRMLPSLsLTEDVKW SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): S158‑p, IRF5 iso2 (human): S158‑p, IRF5 (mouse): S157‑p, IRF5 (rat): S157‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) phosphopeptide analysis
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced
Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.

S293-p - IRF5 (human)
Modsite: VELFGPIsLEQVRFP SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): S293‑p, IRF5 iso2 (human): S309‑p, IRF5 (mouse): T292‑p, IRF5 (rat): T292‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) phosphopeptide analysis

S301-p - IRF5 (human)
Modsite: LEQVRFPsPEDIPSD SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): S301‑p, IRF5 iso2 (human): S317‑p, IRF5 (mouse): S300‑p, IRF5 (rat): S300‑p

S435-p - IRF5 (human)
Modsite: EMFSGELsWSADSIR SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): S435‑p, IRF5 iso2 (human): S451‑p, IRF5 (mouse): S434‑p, IRF5 (rat): S434‑p
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced, transcription, induced
Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.

S446-p - IRF5 (human)
Modsite: DSIRLQIsNPDLKDR SwissProt Entrez-Gene
Orthologous residues
IRF5 (human): S446‑p, IRF5 iso2 (human): S462‑p, IRF5 (mouse): S445‑p, IRF5 (rat): S445‑p
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced, transcription, induced
Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.