Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC (2012) Activation of interferon regulatory factor 5 by site specific phosphorylation. PLoS One 7, e33098 22412986

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T10-p - IRF5 (human) ▲

Modsite: QSIPVAPtPPRRVRL SwissProt Entrez-Gene Orthologous residues IRF5 (human): T10‑p, IRF5 iso2 (human): T10‑p, IRF5 (mouse): P10‑p, IRF5 (rat): T10‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human

S158-p - IRF5 (human) ▲

Modsite: QRMLPSLsLTEDVKW SwissProt Entrez-Gene Orthologous residues IRF5 (human): S158‑p, IRF5 iso2 (human): S158‑p, IRF5 (mouse): S157‑p, IRF5 (rat): S157‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE TBK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (human) phosphopeptide analysis Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  apoptosis, induced Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.

S293-p - IRF5 (human) ▲

Modsite: VELFGPIsLEQVRFP SwissProt Entrez-Gene Orthologous residues IRF5 (human): S293‑p, IRF5 iso2 (human): S309‑p, IRF5 (mouse): T292‑p, IRF5 (rat): T292‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE TBK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (human) phosphopeptide analysis

S301-p - IRF5 (human) ▲

Modsite: LEQVRFPsPEDIPSD SwissProt Entrez-Gene Orthologous residues IRF5 (human): S301‑p, IRF5 iso2 (human): S317‑p, IRF5 (mouse): S300‑p, IRF5 (rat): S300‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human

S435-p - IRF5 (human) ▲

Modsite: EMFSGELsWsADsIR SwissProt Entrez-Gene Orthologous residues IRF5 (human): S435‑p, IRF5 iso2 (human): S451‑p, IRF5 (mouse): S434‑p, IRF5 (rat): S434‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  apoptosis, induced, transcription, induced Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.

S446-p - IRF5 (human) ▲

Modsite: DsIRLQIsNPDLKDR SwissProt Entrez-Gene Orthologous residues IRF5 (human): S446‑p, IRF5 iso2 (human): S462‑p, IRF5 (mouse): S445‑p, IRF5 (rat): S445‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  293 (epithelial), HT1080 (fibroblast) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  apoptosis, induced, transcription, induced Comments:  nuclear localization- S158 requires an activator, while S435 and S446 do not.