Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 19740738
Peck GR, et al. (2009) Insulin-stimulated phosphorylation of the Rab GTPase-activating protein TBC1D1 regulates GLUT4 translocation. J Biol Chem 284, 30016-23 19740738
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S145-p - TBC1D1 (mouse)
Modsite: TKVPEIIssIRQAGK SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S145‑p, TBC1D1 iso2 (human): S145‑p, TBC1D1 (mouse): S145‑p, TBC1D1 iso2 (mouse): S145‑p, TBC1D1 (rat): S145‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S146-p - TBC1D1 (mouse)
Modsite: KVPEIIssIRQAGKI SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S146‑p, TBC1D1 iso2 (human): S146‑p, TBC1D1 (mouse): S146‑p, TBC1D1 iso2 (mouse): S146‑p, TBC1D1 (rat): S146‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S229-p - TBC1D1 (mouse)
Modsite: GPRPMRKsFsQPGLR SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S235‑p, TBC1D1 iso2 (human): S235‑p, TBC1D1 (mouse): S229‑p, TBC1D1 iso2 (mouse): S229‑p, TBC1D1 (rat): S229‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S231-p - TBC1D1 (mouse)
Modsite: RPMRKsFsQPGLRSL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S237‑p, TBC1D1 iso2 (human): S237‑p, TBC1D1 (mouse): S231‑p, TBC1D1 iso2 (mouse): S231‑p, TBC1D1 (rat): S231‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S489-p - TBC1D1 (mouse)
Modsite: ASRFRLDsLKNRAKR SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): , TBC1D1 (mouse): S489‑p, TBC1D1 iso2 (mouse): S489‑p, TBC1D1 (rat): S489‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S497-p - TBC1D1 (mouse)
Modsite: LKNRAKRsLtEsLEs SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S503‑p, TBC1D1 iso2 (human): S503‑p, TBC1D1 (mouse): S497‑p, TBC1D1 iso2 (mouse): S497‑p, TBC1D1 (rat): S497‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T499-p - TBC1D1 (mouse)
Modsite: NRAKRsLtEsLEsIL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T505‑p, TBC1D1 iso2 (human): T505‑p, TBC1D1 (mouse): T499‑p, TBC1D1 iso2 (mouse): T499‑p, TBC1D1 (rat): T499‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Comments:  reduces inhibitory effect on TBC1D1 on GLUT4 translocation

S501-p - TBC1D1 (mouse)
Modsite: AKRsLtEsLEsILSR SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S507‑p, TBC1D1 iso2 (human): S507‑p, TBC1D1 (mouse): S501‑p, TBC1D1 iso2 (mouse): S501‑p, TBC1D1 (rat): S501‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Comments:  reduces inhibitory effect on TBC1D1 on GLUT4 translocation

S519-p - TBC1D1 (mouse)
Modsite: ARGLQDHsAsVDLDS SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S525‑p, TBC1D1 iso2 (human): S525‑p, TBC1D1 (mouse): S519‑p, TBC1D1 iso2 (mouse): S519‑p, TBC1D1 (rat): S519‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S521-p - TBC1D1 (mouse)
Modsite: GLQDHsAsVDLDSST SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S527‑p, TBC1D1 iso2 (human): S527‑p, TBC1D1 (mouse): S521‑p, TBC1D1 iso2 (mouse): S521‑p, TBC1D1 (rat): S521‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S559-p - TBC1D1 (mouse)
Modsite: TSFKLLGssDDLssD SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S565‑p, TBC1D1 iso2 (human): S565‑p, TBC1D1 (mouse): S559‑p, TBC1D1 iso2 (mouse): S559‑p, TBC1D1 (rat): S559‑p

S560-p - TBC1D1 (mouse)
Modsite: SFKLLGssDDLssDs SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S566‑p, TBC1D1 iso2 (human): S566‑p, TBC1D1 (mouse): S560‑p, TBC1D1 iso2 (mouse): S560‑p, TBC1D1 (rat): S560‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S565-p - TBC1D1 (mouse)
Modsite: GssDDLssDsEGHIA SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S571‑p, TBC1D1 iso2 (human): S571‑p, TBC1D1 (mouse): S565‑p, TBC1D1 iso2 (mouse): S565‑p, TBC1D1 (rat): S565‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T590-p - TBC1D1 (mouse)
Modsite: AFRRRANtLsHFPVE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  activity, inhibited
Comments:  reduces inhibitory effect on TBC1D1 on GLUT4 translocation

S608-p - TBC1D1 (mouse)
Modsite: PPEPAQssPGVsQRK SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S614‑p, TBC1D1 iso2 (human): S614‑p, TBC1D1 (mouse): S608‑p, TBC1D1 iso2 (mouse): S608‑p, TBC1D1 (rat): S608‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S621-p - TBC1D1 (mouse)
Modsite: RKLMRYHsVstETPH SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S627‑p, TBC1D1 iso2 (human): S627‑p, TBC1D1 (mouse): S621‑p, TBC1D1 iso2 (mouse): S621‑p, TBC1D1 (rat): S621‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S660-p - TBC1D1 (mouse)
Modsite: PRLNPSAssPNFFKY SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S667‑p, TBC1D1 (mouse): S660‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S662‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S661-p - TBC1D1 (mouse)
Modsite: RLNPSAssPNFFKYL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S668‑p, TBC1D1 (mouse): S661‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S663‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S697-p - TBC1D1 (mouse)
Modsite: ALRKKLHssssVPNF SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S704‑p, TBC1D1 (mouse): S697‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S699‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S698-p - TBC1D1 (mouse)
Modsite: LRKKLHssssVPNFL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S705‑p, TBC1D1 (mouse): S698‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S700‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S699-p - TBC1D1 (mouse)
Modsite: RKKLHssssVPNFLK SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S706‑p, TBC1D1 (mouse): S699‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S701‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S700-p - TBC1D1 (mouse)
Modsite: KKLHssssVPNFLKF SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S707‑p, TBC1D1 (mouse): S700‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S702‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1028-p - TBC1D1 (mouse)
Modsite: QIQMYQLsRLLHDYH SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S941‑p, TBC1D1 iso2 (human): , TBC1D1 (mouse): S1028‑p, TBC1D1 iso2 (mouse): S935‑p, TBC1D1 (rat): S1030‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1039-p - TBC1D1 (mouse)
Modsite: HDYHRDLyNHLEEHE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): Y952‑p, TBC1D1 iso2 (human): , TBC1D1 (mouse): Y1039‑p, TBC1D1 iso2 (mouse): Y946‑p, TBC1D1 (rat): Y1041‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T1218-p - TBC1D1 (mouse)
Modsite: KLKQAALtLEVERSA SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T1131‑p, TBC1D1 iso2 (human): T1122‑p, TBC1D1 (mouse): T1218‑p, TBC1D1 iso2 (mouse): T1125‑p, TBC1D1 (rat): T1220‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse