Curated Information
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Home > Curated Information Page > PubMed Id: 22898603
Spring K, et al. (2012) Tyrosine phosphorylation of DEP-1/CD148 as a mechanism controlling Src kinase activation, endothelial cell permeability, invasion, and capillary formation. Blood 120, 2745-56 22898603
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1311-p - PTPRJ (human)
Modsite: DSkVDLIyQNtTAMt SwissProt Entrez-Gene
Orthologous residues
PTPRJ (human): Y1311‑p, PTPRJ (mouse): Y1212‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of wild-type enzyme
KINASE Src (human) co-immunoprecipitation, transfection of constitutively active enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) SH2 Induces pull-down assay, in vitro

Y1320-p - PTPRJ (human)
Modsite: NtTAMtIyENLAPVT SwissProt Entrez-Gene
Orthologous residues
PTPRJ (human): Y1320‑p, PTPRJ (mouse): Y1221‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of wild-type enzyme
KINASE Src (human) co-immunoprecipitation, transfection of constitutively active enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) SH2 Induces pull-down assay, in vitro

Y419-p - Src (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTPRJ (human) transfection of inactive enzyme, transfection of wild-type enzyme

Y530-p - Src (human)
Modsite: FTstEPQyQPGENL_ SwissProt Entrez-Gene
Orthologous residues
Src (human): Y530‑p, Src iso2 (human): Y536‑p, Src (mouse): Y535‑p, Src iso2 (mouse): Y529‑p, Src (rat): Y530‑p, Src (chicken): Y527‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTPRJ (human) transfection of inactive enzyme, transfection of wild-type enzyme