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Home > Curated Information Page > PubMed Id: 16818236
Lu C, et al. (2006) Cell apoptosis: requirement of H2AX in DNA ladder formation, but not for the activation of caspase-3. Mol Cell 23, 121-32 16818236
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T183-p - JNK1 iso2 (human)
Modsite: AGTSFMMtPyVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse

Y185-p - JNK1 iso2 (human)
Modsite: TSFMMtPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse

T53-p - ATF-2 (mouse)
Modsite: IVADQtPtPtRFLKN SwissProt Entrez-Gene
Orthologous residues
ATF‑2 (human): T71‑p, ATF‑2 (mouse): T53‑p, ATF‑2 (rat): T53‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV P38B (mouse) inhibit treatment-induced increase dominant negative

T55-p - ATF-2 (mouse)
Modsite: ADQtPtPtRFLKNCE SwissProt Entrez-Gene
Orthologous residues
ATF‑2 (human): T73‑p, ATF‑2 (mouse): T55‑p, ATF‑2 (rat): T55‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV P38B (mouse) inhibit treatment-induced increase dominant negative

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

S139-p - H2AX (mouse)
Modsite: GkKAsQAsQEy____ SwissProt Entrez-Gene
Orthologous residues
H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), JB6 RT101 (epidermal), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK2 (mouse)
KINASE JNK1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK2 (mouse) pharmacological activator of upstream enzyme, genetic knockout/knockin of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, microscopy-colocalization, phospho-antibody
KINASE JNK1 (mouse) pharmacological activator of upstream enzyme, siRNA inhibition of enzyme, transfection of dominant-negative enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, microscopy-colocalization, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
SP600125 UV inhibit treatment-induced increase
PD98059 UV no effect upon treatment-induced increase
SB202190 UV no effect upon treatment-induced increase
UV ERK2 (mouse) no effect upon treatment-induced increase dominant negative
UV P38B (mouse) no effect upon treatment-induced increase dominant negative
UV JNK1 (mouse) inhibit treatment-induced increase dominant negative
UV JNK2 (mouse) inhibit treatment-induced increase homozygous knockout
siRNA JNK1 (mouse) JNK2 (mouse) augment treatment-induced decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  apoptosis, induced

S63-p - Jun (mouse)
Modsite: kNsDLLtsPDVGLLK SwissProt Entrez-Gene
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p, Jun (cow): S63‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
JNK1 (mouse) inhibit treatment-induced increase dominant negative