Curated Information
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Home > Curated Information Page > PubMed Id: 7545671
Han JW, Pearson RB, Dennis PB, Thomas G (1995) Rapamycin, wortmannin, and the methylxanthine SQ20006 inactivate p70s6k by inducing dephosphorylation of the same subset of sites. J Biol Chem 270, 21396-403 7545671
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T252-p - p70S6K (human)
Modsite: HDGtVtHtFCGtIEY SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
wortmannin serum inhibit treatment-induced increase
methylxanthine serum inhibit treatment-induced increase
rapamycin serum inhibit treatment-induced increase
FK506 rapamycin inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
wortmannin serum inhibit treatment-induced increase
methylxanthine serum inhibit treatment-induced increase
rapamycin serum inhibit treatment-induced increase
FK506 rapamycin inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S427-p - p70S6K (human)
Modsite: EsVKEkFsFEPKIRs SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S427‑p, p70S6K iso2 (human): S404‑p, p70S6K (mouse): S427‑p, p70S6K (rat): S427‑p, p70S6K iso2 (rat): S404‑p, p70S6K (fruit fly): R413‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
wortmannin serum inhibit treatment-induced increase
methylxanthine serum inhibit treatment-induced increase
rapamycin serum inhibit treatment-induced increase
FK506 rapamycin inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S434-p - p70S6K (human)
Modsite: sFEPKIRsPRRFIGs SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S434‑p, p70S6K iso2 (human): S411‑p, p70S6K (mouse): S434‑p, p70S6K (rat): S434‑p, p70S6K iso2 (rat): S411‑p, p70S6K (fruit fly): S418‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
wortmannin serum inhibit treatment-induced increase
methylxanthine serum inhibit treatment-induced increase
rapamycin serum inhibit treatment-induced increase
FK506 rapamycin inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S441-p - p70S6K (human)
Modsite: sPRRFIGsPRtPVsP SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S441‑p, p70S6K iso2 (human): S418‑p, p70S6K (mouse): S441‑p, p70S6K (rat): S441‑p, p70S6K iso2 (rat): S418‑p, p70S6K (fruit fly): Q425‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
rapamycin serum no effect upon treatment-induced increase
wortmannin serum no effect upon treatment-induced increase
methylxanthine serum no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T444-p - p70S6K (human)
Modsite: RFIGsPRtPVsPVkF SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): S429‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
rapamycin serum no effect upon treatment-induced increase
wortmannin serum no effect upon treatment-induced increase
methylxanthine serum no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S447-p - p70S6K (human)
Modsite: GsPRtPVsPVkFsPG SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): S430‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
rapamycin serum no effect upon treatment-induced increase
wortmannin serum no effect upon treatment-induced increase
methylxanthine serum no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced