Curated Information
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Home > Curated Information Page > PubMed Id: 22802128
Faure C, Ramos M, Girault JA (2013) Pyk2 cytonuclear localization: mechanisms and regulation by serine dephosphorylation. Cell Mol Life Sci 70, 137-52 22802128
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  PC-12 (chromaffin)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) Autophosphorylation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
NKH_477 no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S778-p - Pyk2 (human)
Modsite: HNVFKRHsMREEDFI SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): S778‑p, Pyk2 iso2 (human): , Pyk2 (mouse): S778‑p, Pyk2 (rat): S778‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, immunoassay, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), PC-12 (chromaffin)
Cellular systems studied:  cell lines
Species studied:  green monkey, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
PHOSPHATASE PPP3CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP3CA (human) pharmacological inhibitor of upstream enzyme
KINASE PKACA (human) modification site within consensus motif, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
depolarization decrease
FK506 depolarization inhibit treatment-induced decrease
mPKI decrease
H-89 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization