Curated Information
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Home > Curated Information Page > PubMed Id: 22633953
Schröfelbauer B, et al. (2012) NEMO Ensures Signaling Specificity of the Pleiotropic IKKβ by Directing Its Kinase Activity toward IκBα. Mol Cell 47, 111-21 22633953
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S32-p - IkB-alpha (mouse)
Modsite: LVDDRHDsGLDsMKD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1RA (mouse) increase
IKKG (mouse) increase
SC-514 decrease
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  autophagy, inhibited

S36-p - IkB-alpha (mouse)
Modsite: RHDsGLDsMKDEEYE SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1RA (mouse) increase
IKKG (mouse) increase
SC-514 decrease
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  autophagy, inhibited

S935-p - NFkB-p105 (mouse)
Modsite: CDSGVETsFRKLsFT SwissProt Entrez-Gene
Orthologous residues
NFkB‑p105 (human): S932‑p, NFkB‑p105 iso2 (human): S933‑p, NFkB‑p105 (mouse): S935‑p, NFkB‑p105 (rat): S936‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme

S534-p - NFkB-p65 (mouse)
Modsite: sGDEDFssIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NBD_peptide no change compared to control
SC-514 decrease

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NBD_peptide no change compared to control
SC-514 decrease

S484-p - TSC1 (mouse)
Modsite: AAISKELsEITTAEA SwissProt Entrez-Gene
Orthologous residues
TSC1 (human): S487‑p, TSC1 (mouse): S484‑p, TSC1 iso2 (mouse): S483‑p, TSC1 (rat): S487‑p

S508-p - TSC1 (mouse)
Modsite: DsPFyRDsLsGsQRK SwissProt Entrez-Gene
Orthologous residues
TSC1 (human): S511‑p, TSC1 (mouse): S508‑p, TSC1 iso2 (mouse): S507‑p, TSC1 (rat): S511‑p