Kim JY, et al. (2012) Functional regulation of Slug / Snail2 is dependent on GSK-3β-mediated phosphorylation. FEBS J 279, 2929-39 22727060

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S87-p - Snail2 (human) ▲

Modsite: SSSLGRVsPPPPsDT SwissProt Entrez-Gene Orthologous residues Snail2 (human): S87‑p, Snail2 (mouse): S88‑p, Snail2 (rat): S87‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human

S92-p - Snail2 (human) ▲

Modsite: RVsPPPPsDTSsKDH SwissProt Entrez-Gene Orthologous residues Snail2 (human): S92‑p, Snail2 (mouse): S93‑p, Snail2 (rat): S92‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  transcription, induced Comments:  transcriptional activity of E-cadherin; WT localizes in cytosol and nucleus; mutants enhance epithelial-mesenchymal transition (EMT)

S96-p - Snail2 (human) ▲

Modsite: PPPsDTSsKDHsGSE SwissProt Entrez-Gene Orthologous residues Snail2 (human): S96‑p, Snail2 (mouse): S97‑p, Snail2 (rat): S96‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  transcription, induced Comments:  transcriptional activity of E-cadherin; WT localized in nucleus and cytoplasm, mutants localize in nucleus; mutants enhance epithelial-mesenchymal transition (EMT), transcriptional activity of E-cadherin; WT localizes in cytosol and nucleus; mutants enhance epithelial-mesenchymal transition (EMT)

S100-p - Snail2 (human) ▲

Modsite: DTSsKDHsGSEsPIS SwissProt Entrez-Gene Orthologous residues Snail2 (human): S100‑p, Snail2 (mouse): S101‑p, Snail2 (rat): S100‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  transcription, induced Comments:  transcriptional activity of E-cadherin; WT localized in nucleus and cytoplasm, mutants localize in nucleus; mutants enhance epithelial-mesenchymal transition (EMT)

S104-p - Snail2 (human) ▲

Modsite: KDHsGSEsPISDEEE SwissProt Entrez-Gene Orthologous residues Snail2 (human): S104‑p, Snail2 (mouse): S105‑p, Snail2 (rat): S104‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif