Curated Information
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Home > Curated Information Page > PubMed Id: 22609160
Li J, et al. (2012) Grp1 plays a key role in linking insulin signaling to glut4 recycling. Dev Cell 22, 1286-98 22609160
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S155-p - cytohesin 3 (mouse)
Modsite: ALRQFLWsFRLPGEA SwissProt Entrez-Gene
Orthologous residues
cytohesin 3 (human): S155‑p, cytohesin 3 (mouse): S155‑p, cytohesin 3 (rat): S155‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) phospho-antibody, phospho-motif antibody, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA decrease siRNA for Akt decreases phosphorylation
LY294002 decrease
ATP increase
Downstream Regulation
Effect of modification (function):  receptor recycling, induced
Comments:  ARF6 activation, Glut4 vesicle formation

T280-p - cytohesin 3 (mouse)
Modsite: KLGGRVKtWKRRWFI SwissProt Entrez-Gene
Orthologous residues
cytohesin 3 (human): T281‑p, cytohesin 3 (mouse): T280‑p, cytohesin 3 (rat): T281‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) phospho-antibody, phospho-motif antibody, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA decrease siRNA for Akt decreases phosphorylation
LY294002 decrease
ATP increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, receptor recycling, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GLUT4 (human) Induces pull-down assay
Comments:  Glut4 vesicle formation