Curated Information
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Home > Curated Information Page > PubMed Id: 22581287
Sheng L, et al. (2012) NF-κB–inducing kinase (NIK) promotes hyperglycemia and glucose intolerance in obesity by augmenting glucagon action. Nat Med 18, 943-9 22581287
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S94-p - CREB (mouse)
Modsite: QISTIAEsEDsQEsV SwissProt Entrez-Gene
Orthologous residues
CREB (human): S94‑p, CREB iso1 (human): S108‑p, CREB (mouse): S94‑p, CREB iso1 (mouse): S108‑p, CREB (rat): S108‑p

S97-p - CREB (mouse)
Modsite: TIAEsEDsQEsVDsV SwissProt Entrez-Gene
Orthologous residues
CREB (human): S97‑p, CREB iso1 (human): S111‑p, CREB (mouse): S97‑p, CREB iso1 (mouse): S111‑p, CREB (rat): S111‑p

S100-p - CREB (mouse)
Modsite: EsEDsQEsVDsVTDs SwissProt Entrez-Gene
Orthologous residues
CREB (human): S100‑p, CREB iso1 (human): S114‑p, CREB (mouse): S100‑p, CREB iso1 (mouse): S114‑p, CREB (rat): S114‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  hepatocyte-liver, HepG2 (hepatic)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Nik (human)

S103-p - CREB (mouse)
Modsite: DsQEsVDsVTDsQKR SwissProt Entrez-Gene
Orthologous residues
CREB (human): S103‑p, CREB iso1 (human): S117‑p, CREB (mouse): S103‑p, CREB iso1 (mouse): S117‑p, CREB (rat): S117‑p

S107-p - CREB (mouse)
Modsite: sVDsVTDsQKRREIL SwissProt Entrez-Gene
Orthologous residues
CREB (human): S107‑p, CREB iso1 (human): S121‑p, CREB (mouse): S107‑p, CREB iso1 (mouse): S121‑p, CREB (rat): S121‑p

S115-p - CREB (mouse)
Modsite: QKRREILsRRPsyRk SwissProt Entrez-Gene
Orthologous residues
CREB (human): S115‑p, CREB iso1 (human): S129‑p, CREB (mouse): S115‑p, CREB iso1 (mouse): S129‑p, CREB (rat): S129‑p

S119-p - CREB (mouse)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S133‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Nik (mouse) transfection of wild-type enzyme, transfection of inactive enzyme, genetic knockout/knockin of upstream enzyme

S128-p - CREB (mouse)
Modsite: RkILNDLssDAPGVP SwissProt Entrez-Gene
Orthologous residues
CREB (human): S128‑p, CREB iso1 (human): S142‑p, CREB (mouse): S128‑p, CREB iso1 (mouse): S142‑p, CREB (rat): S142‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  hepatocyte-liver, HepG2 (hepatic)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Nik (human)

S129-p - CREB (mouse)
Modsite: kILNDLssDAPGVPR SwissProt Entrez-Gene
Orthologous residues
CREB (human): S129‑p, CREB iso1 (human): S143‑p, CREB (mouse): S129‑p, CREB iso1 (mouse): S143‑p, CREB (rat): S143‑p

S142-p - CREB (mouse)
Modsite: PRIEEEKsEEETSAP SwissProt Entrez-Gene
Orthologous residues
CREB (human): S142‑p, CREB iso1 (human): S156‑p, CREB (mouse): S142‑p, CREB iso1 (mouse): S156‑p, CREB (rat): S156‑p

S257-p - CREB (mouse)
Modsite: PGVVMAssPALPTQP SwissProt Entrez-Gene
Orthologous residues
CREB (human): S257‑p, CREB iso1 (human): S271‑p, CREB (mouse): S257‑p, CREB iso1 (mouse): S271‑p, CREB (rat): S271‑p

S326-p - CREB (mouse)
Modsite: KDLYCHKsD______ SwissProt Entrez-Gene
Orthologous residues
CREB (human): S326‑p, CREB iso1 (human): S340‑p, CREB (mouse): S326‑p, CREB iso1 (mouse): S340‑p, CREB (rat): S340‑p

S98-p - CREB iso1 (mouse)
Modsite: KDLKRLFsGtQISTI SwissProt Entrez-Gene
Orthologous residues
CREB (human): , CREB iso1 (human): S98‑p, CREB (mouse): , CREB iso1 (mouse): S98‑p, CREB (rat): S98‑p

T100-p - CREB iso1 (mouse)
Modsite: LKRLFsGtQISTIAE SwissProt Entrez-Gene
Orthologous residues
CREB (human): , CREB iso1 (human): T100‑p, CREB (mouse): , CREB iso1 (mouse): T100‑p, CREB (rat): T100‑p