Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22561688
Wang S, et al. (2012) Activation of AMP-activated protein kinase α2 by nicotine instigates formation of abdominal aortic aneurysms in mice in vivo. Nat Med 18, 902-10 22561688
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S80-p - ACC1 (human)
Modsite: LHIRssMsGLHLVkQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  aorta, blood, VSMC-aorta
Cellular systems studied:  primary cultured cells, tissue
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
nicotine increase

T172-p - AMPKA2 (human)
Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  aorta, blood, VSMC-aorta
Cellular systems studied:  primary cultured cells, tissue
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
nicotine increase
Downstream Regulation
Effect of modification (function):  intracellular localization, phosphorylation

S219-p - AP-2 alpha (human)
Modsite: CSVPGRLsLLSsTsk SwissProt Entrez-Gene
Orthologous residues
AP‑2 alpha (human): S219‑p, AP‑2 alpha iso2 (human): S213‑p, AP‑2 alpha (mouse): S219‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), VSMC-aorta
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA2 (human) co-immunoprecipitation, pharmacological activator of upstream enzyme, siRNA inhibition of enzyme, microscopy-colocalization, modification site within consensus motif, transfection of wild-type enzyme, transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, activation of upstream enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotine increase
angiotensin_2 increase
compound_C angiotensin_2, nicotine inhibit treatment-induced increase
siRNA decrease siRNA for AMPKA2 decreases phosphorylation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces