Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22510297
Tsai YR, et al. (2012) The signaling mechanisms mediating the inhibitory effect of TCH-1116 on formyl peptide-stimulated superoxide anion generation in neutrophils. Eur J Pharmacol 682, 171-80 22510297
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
TCH-116 fMLP inhibit treatment-induced increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
TCH-116 fMLP inhibit treatment-induced increase

S144-p - PAK1 (rat)
Modsite: SNSQKYMsFTDKsAE SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S144‑p, PAK1 (mouse): S144‑p, PAK1 (rat): S144‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
TCH-116 fMLP no effect upon treatment-induced increase

T422-p - PAK1 (rat)
Modsite: PEQSKRstMVGTPYW SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): T423‑p, PAK1 (mouse): T423‑p, PAK1 (rat): T422‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
TCH-116 fMLP inhibit treatment-induced increase

T402-p - PAK2 (rat)
Modsite: PEQSKRstMVGTPYW SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): T402‑p, PAK2 (mouse): T402‑p, PAK2 (rat): T402‑p, PAK2 (rabbit): T402‑p, PAK2 (cow): T402‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
TCH-116 fMLP inhibit treatment-induced increase

S244-p - PDK1 (rat)
Modsite: SKQARANsFVGTAQY SwissProt Entrez-Gene
Orthologous residues
PDK1 (human): S241‑p, PDK1 iso2 (human): S191‑p, PDK1 iso4 (human): S114‑p, PDK1 iso5 (human): S241‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil-blood
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP no change compared to control
TCH-116 fMLP no change compared to control