Curated Information
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Home > Curated Information Page > PubMed Id: 22629392
Wang S, et al. (2012) Extensive Crosstalk between O-GlcNAcylation and Phosphorylation Regulates Akt Signaling. PLoS One 7, e37427 22629392
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S126-gl - Akt1 (human)
Modsite: DFRsGsPsDNsGAEE SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S126‑gl, Akt1 iso2 (human): S64‑gl, Akt1 (mouse): S126‑gl, Akt1 (rat): S126‑gl, Akt1 (fruit fly): A238‑gl, Akt1 (cow): G126‑gl
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase

S129-gl - Akt1 (human)
Modsite: sGsPsDNsGAEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S129‑gl, Akt1 iso2 (human): S67‑gl, Akt1 (mouse): S129‑gl, Akt1 (rat): S129‑gl, Akt1 (fruit fly): E241‑gl, Akt1 (cow): S129‑gl
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase

T305-gl - Akt1 (human)
Modsite: EGIkDGAtMKtFCGt SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T305‑gl, Akt1 iso2 (human): T243‑gl, Akt1 (mouse): T305‑gl, Akt1 (rat): T305‑gl, Akt1 (fruit fly): T420‑gl, Akt1 (cow): T305‑gl
Characterization
Methods used to characterize site in vivo mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
Comments:  suppresses Akt phosphorylation at T308 by disrupting its interaction with PDK1

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc decrease
Akt1 (human) decrease Akt T305A and T312A glycosylation mutants

T312-gl - Akt1 (human)
Modsite: tMKtFCGtPEyLAPE SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T312‑gl, Akt1 iso2 (human): T250‑gl, Akt1 (mouse): T312‑gl, Akt1 (rat): T312‑gl, Akt1 (fruit fly): T427‑gl, Akt1 (cow): T312‑gl
Characterization
Methods used to characterize site in vivo mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
Comments:  suppresses Akt phosphorylation at T308 by disrupting its interaction with PDK1

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc decrease