Curated Information
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Home > Curated Information Page > PubMed Id: 10066810
Wang Y, Guo W, Liang L, Esselman WJ (1999) Phosphorylation of CD45 by casein kinase 2. Modulation of activity and mutational analysis. J Biol Chem 274, 7454-61 10066810
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S990-p - CD45 (mouse)
Modsite: SKESEPEsDEssDDD SwissProt Entrez-Gene
Orthologous residues
CD45 (human): S999‑p, CD45 (mouse): S990‑p, CD45 (rat): S970‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  CTLL-2 (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (mouse)
KINASE CK2A1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (mouse) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S993-p - CD45 (mouse)
Modsite: SEPEsDEssDDDsDS SwissProt Entrez-Gene
Orthologous residues
CD45 (human): S1002‑p, CD45 (mouse): S993‑p, CD45 (rat): S973‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  CTLL-2 (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (mouse)
KINASE CK2A1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (mouse) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S994-p - CD45 (mouse)
Modsite: EPEsDEssDDDsDSE SwissProt Entrez-Gene
Orthologous residues
CD45 (human): S1003‑p, CD45 (mouse): S994‑p, CD45 (rat): S974‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  CTLL-2 (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (mouse)
PHOSPHATASE PPP2CA (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (mouse) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S998-p - CD45 (mouse)
Modsite: DEssDDDsDSEETSK SwissProt Entrez-Gene
Orthologous residues
CD45 (human): S1007‑p, CD45 (mouse): S998‑p, CD45 (rat): S978‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  CTLL-2 (T lymphocyte), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (mouse)
KINASE CK2A1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (mouse) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced