Limesand KH, Schwertfeger KL, Anderson SM (2006) MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells. Mol Cell Biol 26, 8840-56 16982679

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S345-p - Chk1 (mouse) ▲

Modsite: LVQGISFsQPTCPEH SwissProt Entrez-Gene Orthologous residues Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)] Cellular systems studied:  primary cells, tissue Species studied:  mouse Comments:  +/- myr-Akt1 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide Akt1 (mouse) no change compared to control constitutively active siRNA etoposide MDM2 (mouse) Akt1 (mouse) increase siRNA MDM2 (mouse) increase in constitutively active Akt1-expressing cells

S21-p - GSK3A (mouse) ▲

Modsite: sGRARtssFAEPGGG SwissProt Entrez-Gene Orthologous residues GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)] Cellular systems studied:  primary cells, tissue Species studied:  mouse Comments:  +/- myr-Akt1 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Akt1 (mouse) increase constitutively active

S9-p - GSK3B (mouse) ▲

Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene Orthologous residues GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)] Cellular systems studied:  primary cells, tissue Species studied:  mouse Comments:  +/- myr-Akt1 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Akt1 (mouse) increase constitutively active

S163-p - MDM2 (mouse) ▲

Modsite: SSRRRsIsEtEENTD SwissProt Entrez-Gene Orthologous residues MDM2 (human): S166‑p, MDM2 (mouse): S163‑p, MDM2 (rat): S138‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)] Cellular systems studied:  primary cells, tissue Species studied:  mouse Comments:  +/- myr-Akt1 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Akt1 (mouse) increase constitutively active Akt1 (mouse) decrease dominant negative

S18-p - p53 (mouse) ▲

Modsite: IsLELPLsQEtFsGL SwissProt Entrez-Gene Orthologous residues p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): ‑, p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)] Cellular systems studied:  primary cells, tissue Species studied:  mouse Comments:  +/- myr-Akt1 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ionizing_radiation increase ionizing_radiation Akt1 (mouse) inhibit treatment-induced increase constitutively active etoposide increase etoposide Akt1 (mouse) inhibit treatment-induced increase constitutively active siRNA MDM2 (human) Akt1 (mouse) inhibit treatment-induced decrease