Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 16980435
Suzuki H, et al. (2006) Activation of endothelial nitric oxide synthase by the angiotensin II type 1 receptor. Endocrinology 147, 5914-20 16980435
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S154-p - VASP (rat)
Modsite: EHLERRVsNAGGPPA SwissProt Entrez-Gene
Orthologous residues
VASP (human): S157‑p, VASP (mouse): S153‑p, VASP (rat): S154‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), vascular smooth muscle cell ('muscle, smooth') [eNOS (cow)]
Cellular systems studied:  cell lines
Species studied:  cow, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
angiotensin_2 eNOS (human) augment treatment-induced increase

S236-p - VASP (rat)
Modsite: GAKLRKVsKEEASGG SwissProt Entrez-Gene
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), vascular smooth muscle cell ('muscle, smooth') [eNOS (cow)]
Cellular systems studied:  cell lines
Species studied:  cow, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
angiotensin_2 eNOS (human) augment treatment-induced increase

T497-p - eNOS (cow)
Modsite: AGITRKktFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), vascular smooth muscle cell ('muscle, smooth') [eNOS (cow)]
Cellular systems studied:  cell lines
Species studied:  cow, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 no change compared to control

T1177-p - eNOS (cow)
Modsite: EVTSRIRtQsFsLQE SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T1175‑p, eNOS (mouse): T1174‑p, eNOS (rat): T1174‑p, eNOS (rabbit): T1181‑p, eNOS (pig): T1177‑p, eNOS (cow): T1177‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), vascular smooth muscle cell ('muscle, smooth') [eNOS (cow)]
Cellular systems studied:  cell lines
Species studied:  cow, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
RNH6270 angiotensin_2 inhibit treatment-induced increase
YM-254890 angiotensin_2 inhibit treatment-induced increase
L-NAME angiotensin_2 inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  stimulates VASP S236 phosphorylation in the presence of angiotensin 2 (AngII)