Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22516431
Chao A, et al. (2012) Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin. J Proteomics 75, 3342-50 22516431
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S20-p - MYPT1 (human)
Modsite: QLKRWIGsEtDLEPP SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S20‑p, MYPT1 iso2 (human): S20‑p, MYPT1 iso3 (human): S20‑p, MYPT1 iso4 (human): S20‑p, MYPT1 (mouse): S20‑p, MYPT1 (rat): S20‑p, MYPT1 (pig): S20‑p, MYPT1 (chicken): S20‑p, MYPT1 (cow): S20‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

T22-p - MYPT1 (human)
Modsite: KRWIGsEtDLEPPVV SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T22‑p, MYPT1 iso2 (human): T22‑p, MYPT1 iso3 (human): T22‑p, MYPT1 iso4 (human): T22‑p, MYPT1 (mouse): T22‑p, MYPT1 (rat): T22‑p, MYPT1 (pig): T22‑p, MYPT1 (chicken): T22‑p, MYPT1 (cow): T22‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster

S299-p - MYPT1 (human)
Modsite: sEKRDKKsPLIEsTA SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S299‑p, MYPT1 iso2 (human): S299‑p, MYPT1 iso3 (human): S299‑p, MYPT1 iso4 (human): S299‑p, MYPT1 (mouse): S299‑p, MYPT1 (rat): S299‑p, MYPT1 (pig): S299‑p, MYPT1 (chicken): S299‑p, MYPT1 (cow): S299‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S304-p - MYPT1 (human)
Modsite: KKsPLIEsTANMDNN SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S304‑p, MYPT1 iso2 (human): S304‑p, MYPT1 iso3 (human): S304‑p, MYPT1 iso4 (human): S304‑p, MYPT1 (mouse): S304‑p, MYPT1 (rat): S304‑p, MYPT1 (pig): S304‑p, MYPT1 (chicken): S304‑p, MYPT1 (cow): S304‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S422-p - MYPT1 (human)
Modsite: PttAtKIsPKEEERK SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S422‑p, MYPT1 iso2 (human): S422‑p, MYPT1 iso3 (human): S422‑p, MYPT1 iso4 (human): S422‑p, MYPT1 (mouse): S422‑p, MYPT1 (rat): S422‑p, MYPT1 (pig): S422‑p, MYPT1 (chicken): S417‑p, MYPT1 (cow): S422‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

T443-p - MYPT1 (human)
Modsite: WRLGLRktGsyGALA SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T443‑p, MYPT1 iso2 (human): T443‑p, MYPT1 iso3 (human): T443‑p, MYPT1 iso4 (human): T443‑p, MYPT1 (mouse): T443‑p, MYPT1 (rat): T443‑p, MYPT1 (pig): T443‑p, MYPT1 (chicken): T438‑p, MYPT1 (cow): T443‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S445-p - MYPT1 (human)
Modsite: LGLRktGsyGALAEI SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S445‑p, MYPT1 iso2 (human): S445‑p, MYPT1 iso3 (human): S445‑p, MYPT1 iso4 (human): S445‑p, MYPT1 (mouse): S445‑p, MYPT1 (rat): S445‑p, MYPT1 (pig): S445‑p, MYPT1 (chicken): S440‑p, MYPT1 (cow): S445‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

T453-p - MYPT1 (human)
Modsite: yGALAEItASkEGQk SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T453‑p, MYPT1 iso2 (human): T453‑p, MYPT1 iso3 (human): T453‑p, MYPT1 iso4 (human): T453‑p, MYPT1 (mouse): S453‑p, MYPT1 (rat): T453‑p, MYPT1 (pig): T453‑p, MYPT1 (chicken): T448‑p, MYPT1 (cow): T453‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster

S477-p - MYPT1 (human)
Modsite: sAssPRLsssLDNKE SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S477‑p, MYPT1 iso2 (human): S477‑p, MYPT1 iso3 (human): S477‑p, MYPT1 iso4 (human): S477‑p, MYPT1 (mouse): S477‑p, MYPT1 (rat): S477‑p, MYPT1 (pig): S477‑p, MYPT1 (chicken): S472‑p, MYPT1 (cow): S477‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S478-p - MYPT1 (human)
Modsite: AssPRLsssLDNKEK SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S478‑p, MYPT1 iso2 (human): S478‑p, MYPT1 iso3 (human): S478‑p, MYPT1 iso4 (human): S478‑p, MYPT1 (mouse): S478‑p, MYPT1 (rat): S478‑p, MYPT1 (pig): S478‑p, MYPT1 (chicken): S473‑p, MYPT1 (cow): S478‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S507-p - MYPT1 (human)
Modsite: tIPRRLAstsDIEEK SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S507‑p, MYPT1 iso2 (human): S507‑p, MYPT1 iso3 (human): S507‑p, MYPT1 iso4 (human): S507‑p, MYPT1 (mouse): S507‑p, MYPT1 (rat): S507‑p, MYPT1 (pig): S507‑p, MYPT1 (chicken): S502‑p, MYPT1 (cow): S507‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T508-p - MYPT1 (human)
Modsite: IPRRLAstsDIEEKE SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T508‑p, MYPT1 iso2 (human): T508‑p, MYPT1 iso3 (human): T508‑p, MYPT1 iso4 (human): T508‑p, MYPT1 (mouse): T508‑p, MYPT1 (rat): T508‑p, MYPT1 (pig): T508‑p, MYPT1 (chicken): T503‑p, MYPT1 (cow): T508‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster

S509-p - MYPT1 (human)
Modsite: PRRLAstsDIEEKEN SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S509‑p, MYPT1 iso2 (human): S509‑p, MYPT1 iso3 (human): S509‑p, MYPT1 iso4 (human): S509‑p, MYPT1 (mouse): S509‑p, MYPT1 (rat): S509‑p, MYPT1 (pig): S509‑p, MYPT1 (chicken): S504‑p, MYPT1 (cow): S509‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin decrease

S668-p - MYPT1 (human)
Modsite: EVRERRRsyLtPVRD SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S668‑p, MYPT1 iso2 (human): S668‑p, MYPT1 iso3 (human): S612‑p, MYPT1 iso4 (human): S609‑p, MYPT1 (mouse): S666‑p, MYPT1 (rat): S669‑p, MYPT1 (pig): S668‑p, MYPT1 (chicken): S667‑p, MYPT1 (cow): S668‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T671-p - MYPT1 (human)
Modsite: ERRRsyLtPVRDEEs SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T671‑p, MYPT1 iso2 (human): T671‑p, MYPT1 iso3 (human): T615‑p, MYPT1 iso4 (human): T612‑p, MYPT1 (mouse): T669‑p, MYPT1 (rat): T672‑p, MYPT1 (pig): T671‑p, MYPT1 (chicken): T670‑p, MYPT1 (cow): T671‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S678-p - MYPT1 (human)
Modsite: tPVRDEEsEsQRKAR SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S678‑p, MYPT1 iso2 (human): S678‑p, MYPT1 iso3 (human): S622‑p, MYPT1 iso4 (human): S619‑p, MYPT1 (mouse): S676‑p, MYPT1 (rat): S679‑p, MYPT1 (pig): S678‑p, MYPT1 (chicken): S677‑p, MYPT1 (cow): S678‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S680-p - MYPT1 (human)
Modsite: VRDEEsEsQRKARSR SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S680‑p, MYPT1 iso2 (human): S680‑p, MYPT1 iso3 (human): S624‑p, MYPT1 iso4 (human): S621‑p, MYPT1 (mouse): S678‑p, MYPT1 (rat): S681‑p, MYPT1 (pig): S680‑p, MYPT1 (chicken): S679‑p, MYPT1 (cow): S680‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster

S695-p - MYPT1 (human)
Modsite: QARQsRRstQGVtLt SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S695‑p, MYPT1 iso2 (human): S695‑p, MYPT1 iso3 (human): S639‑p, MYPT1 iso4 (human): S636‑p, MYPT1 (mouse): S693‑p, MYPT1 (rat): S696‑p, MYPT1 (pig): S695‑p, MYPT1 (chicken): S694‑p, MYPT1 (cow): S695‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S871-p - MYPT1 (human)
Modsite: ENEQEQQsDtEEGsN SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S871‑p, MYPT1 iso2 (human): S871‑p, MYPT1 iso3 (human): S815‑p, MYPT1 iso4 (human): S812‑p, MYPT1 (mouse): S870‑p, MYPT1 (rat): S873‑p, MYPT1 (pig): , MYPT1 (chicken): S868‑p, MYPT1 (cow): S869‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster

S910-p - MYPT1 (human)
Modsite: sLLGRsGsysyLEER SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S910‑p, MYPT1 iso2 (human): S910‑p, MYPT1 iso3 (human): S854‑p, MYPT1 iso4 (human): S851‑p, MYPT1 (mouse): S909‑p, MYPT1 (rat): S912‑p, MYPT1 (pig): , MYPT1 (chicken): S909‑p, MYPT1 (cow): S908‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S995-p - MYPT1 (human)
Modsite: RALERRIsEMEEELk SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S995‑p, MYPT1 iso2 (human): S960‑p, MYPT1 iso3 (human): S939‑p, MYPT1 iso4 (human): S936‑p, MYPT1 (mouse): S994‑p, MYPT1 (rat): S997‑p, MYPT1 (pig): , MYPT1 (chicken): G994‑p, MYPT1 (cow): T993‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control