Curated Information
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Home > Curated Information Page > PubMed Id: 22469974
Griner EM, Churchill ME, Brautigan DL, Theodorescu D (2013) PKCĪ± phosphorylation of RhoGDI2 at Ser31 disrupts interactions with Rac1 and decreases GDI activity. Oncogene 32, 1010-7 22469974
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S20-p - RhoGDI beta (human)
Modsite: DDDDELdskLNykPP SwissProt Entrez-Gene
Orthologous residues
RhoGDI beta (human): S20‑p, RhoGDI beta (mouse): S19‑p, RhoGDI beta (rat): S19‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S31-p - RhoGDI beta (human)
Modsite: ykPPPQksLkELQEM SwissProt Entrez-Gene
Orthologous residues
RhoGDI beta (human): S31‑p, RhoGDI beta (mouse): S30‑p, RhoGDI beta (rat): S30‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
GF109203X phorbol_ester inhibit treatment-induced increase
Go_6976 phorbol_ester inhibit treatment-induced increase
siRNA phorbol_ester inhibit treatment-induced increase PKCA siRNA
Downstream Regulation
Effect of modification (function):  activity, inhibited, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RAC1 (human) Disrupts intracellular localization co-immunoprecipitation
Comments:  impaired GDI2 function