Ho PC, et al. (2012) NF-κB-mediated degradation of the coactivator RIP140 regulates inflammatory responses and contributes to endotoxin tolerance. Nat Immunol 13, 379-86 22388040

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y364-p - RIP140 (mouse) ▲

Modsite: SsPKNTSyKNSLERN SwissProt Entrez-Gene Orthologous residues RIP140 (human): Y362‑p, RIP140 (mouse): Y364‑p, RIP140 (rat): Y365‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-peritoneum, RAW 264 (macrophage) Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Syk (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Syk (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Comments:  regulates inflamatory responses and contributes to endotoxin tolerance

Y418-p - RIP140 (mouse) ▲

Modsite: TPTTIDEySDNNPSF SwissProt Entrez-Gene Orthologous residues RIP140 (human): Y417‑p, RIP140 (mouse): Y418‑p, RIP140 (rat): Y419‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-peritoneum, RAW 264 (macrophage) Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Syk (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Syk (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Comments:  regulates inflamatory responses and contributes to endotoxin tolerance

Y436-p - RIP140 (mouse) ▲

Modsite: sSGDESSySNCVPID SwissProt Entrez-Gene Orthologous residues RIP140 (human): Y435‑p, RIP140 (mouse): Y436‑p, RIP140 (rat): Y437‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-peritoneum, RAW 264 (macrophage) Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Syk (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Syk (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Comments:  regulates inflamatory responses and contributes to endotoxin tolerance