Curated Information
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Home > Curated Information Page > PubMed Id: 22139837
Fang Z, et al. (2012) Androgen Receptor Enhances p27 Degradation in Prostate Cancer Cells through Rapid and Selective TORC2 Activation. J Biol Chem 287, 2090-8 22139837
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  CWR22Rv1 (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human

T157-p - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  CWR22Rv1 (prostate cell), LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) activation of upstream enzyme, phospho-antibody, pharmacological inhibitor of upstream enzyme, phospho-motif antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
androstanolone increase
Akt_inhibitor_VIII decrease
Downstream Regulation
Effect of modification (function):  protein degradation

T187-p - p27Kip1 (human)
Modsite: NAGsVEQtPKKPGLR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  CWR22Rv1 (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p