Henriksson E, et al. (2012) The AMPK-related kinase SIK2 is regulated by cAMP via phosphorylation at Ser358 in adipocytes. Biochem J 444, 503-14 22462548

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

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S90-p - QIK (human) ▲

Modsite: YQVMETKsMLYLVTE SwissProt Entrez-Gene Orthologous residues QIK (human): S90‑p, QIK (mouse): S90‑p, QIK (rat): S90‑p Characterization Methods used to characterize site in vivo:  phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S343-p - QIK (human) ▲

Modsite: RLKSHRssFPVEQRL SwissProt Entrez-Gene Orthologous residues QIK (human): S343‑p, QIK (mouse): S343‑p, QIK (rat): S343‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S358-p - QIK (human) ▲

Modsite: DGRQRRPstIAEQTV SwissProt Entrez-Gene Orthologous residues QIK (human): S358‑p, QIK (mouse): S358‑p, QIK (rat): S358‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, phospho-motif antibody, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase CL316,243 increase insulin no change compared to control H-89 decrease Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 beta (human) Induces far-Western, pull-down assay, co-immunoprecipitation Comments:  regulates localization in adipocytes

T484-p - QIK (human) ▲

Modsite: RSGQRRHtLsEVTNQ SwissProt Entrez-Gene Orthologous residues QIK (human): T484‑p, QIK (mouse): T484‑p, QIK (rat): T484‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S512-p - QIK (human) ▲

Modsite: NDSPSLDsVDSEYDM SwissProt Entrez-Gene Orthologous residues QIK (human): S512‑p, QIK (mouse): S512‑p, QIK (rat): S512‑p Characterization Methods used to characterize site in vivo:  phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S534-p - QIK (human) ▲

Modsite: NFLEDNPsLkDIMLA SwissProt Entrez-Gene Orthologous residues QIK (human): S534‑p, QIK (mouse): S534‑p, QIK (rat): S534‑p Characterization Methods used to characterize site in vivo:  phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S576-p - QIK (human) ▲

Modsite: KREVHNRsPVSFREG SwissProt Entrez-Gene Orthologous residues QIK (human): S576‑p, QIK (mouse): S576‑p, QIK (rat): S575‑p Characterization Methods used to characterize site in vivo:  phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S587-p - QIK (human) ▲

Modsite: FREGRRAsDtsLTQG SwissProt Entrez-Gene Orthologous residues QIK (human): S587‑p, QIK (mouse): S587‑p, QIK (rat): S586‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), 293 (epithelial), adipocyte-adipose tissue Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse, rat

S575-p - SIK (human) ▲

Modsite: FQEGRRAsDtSLTQG SwissProt Entrez-Gene Orthologous residues SIK (human): S575‑p, SIK (mouse): S577‑p, SIK (rat): S577‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization