Liu J, et al. (2013) Phosphorylation of Mps1 by BRAFV600E prevents Mps1 degradation and contributes to chromosome instability in melanoma. Oncogene 32, 713-23 22430208

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S281-p - TTK (human) ▲

Modsite: VPVNLLNsPDCDVkt SwissProt Entrez-Gene Orthologous residues TTK (human): S281‑p, TTK iso2 (human): S281‑p, TTK (mouse): S277‑p, TTK iso2 (mouse): S277‑p, TTK (rat): S277‑p, TTK (frog): S283‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Sbcl2 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE BRAF (human) phosphopeptide analysis, pharmacological inhibitor of upstream enzyme, phospho-antibody, mutation in upstream enzyme recognition motif Downstream Regulation Effect of modification (function):  protein stabilization, ubiquitination Effect of modification (process):  carcinogenesis, induced, cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDC27 (human) Disrupts co-immunoprecipitation Comments:  accumulation at centrosomes, aneuploidy, correlated with p-ERK, in human metastatic melanoma, centrosome reduplication

S436-p - TTK (human) ▲

Modsite: VFSVskQsPPIsTSk SwissProt Entrez-Gene Orthologous residues TTK (human): S436‑p, TTK iso2 (human): S435‑p, TTK (mouse): S435‑p, TTK iso2 (mouse): S409‑p, TTK (rat): S414‑p, TTK (frog): S459‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Sbcl2 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE BRAF (human) phosphopeptide analysis, pharmacological inhibitor of upstream enzyme, phospho-antibody, mutation in upstream enzyme recognition motif Downstream Regulation Effect of modification (process):  cytoskeletal reorganization Comments:  centrosome reduplication

S821-p - TTK (human) ▲

Modsite: GQLVGLNsPNsILkA SwissProt Entrez-Gene Orthologous residues TTK (human): S821‑p, TTK iso2 (human): S820‑p, TTK (mouse): S820‑p, TTK iso2 (mouse): S794‑p, TTK (rat): S799‑p, TTK (frog): S844‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Sbcl2 (melanocyte) Cellular systems studied:  cell lines Species studied:  human