Meiri D, et al. (2012) Mechanistic insight into the microtubule and actin cytoskeleton coupling through dynein-dependent RhoGEF inhibition. Mol Cell 45, 642-55 22405273

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S143-p - ARHGEF2 (mouse) ▲

Modsite: GsRRGLssLSLAKSV SwissProt Entrez-Gene Orthologous residues ARHGEF2 (human): S143‑p, ARHGEF2 iso2 (human): S143‑p, ARHGEF2 iso3 (human): S116‑p, ARHGEF2 (mouse): S143‑p, ARHGEF2 (rat): S143‑p, ARHGEF2 (pig): P116‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays DYNLT1 (mouse) Disrupts co-immunoprecipitation Comments:  reduced microtubule localization in S143D mutnt

S885-p - ARHGEF2 (mouse) ▲

Modsite: PLDPRRRsLPAGDAL SwissProt Entrez-Gene Orthologous residues ARHGEF2 (human): S886‑p, ARHGEF2 iso2 (human): S885‑p, ARHGEF2 iso3 (human): S858‑p, ARHGEF2 (mouse): S885‑p, ARHGEF2 (rat): S885‑p, ARHGEF2 (pig): S860‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) mutation in upstream enzyme recognition motif, inhibition of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H-89 decrease Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  cytoskeletal reorganization

T94-p - DYNLT1 (mouse) ▲

Modsite: SSTDGSCtVRWENkT SwissProt Entrez-Gene Orthologous residues DYNLT1 (human): T94‑p, DYNLT1 (mouse): T94‑p, DYNLT1 (rat): T94‑p, DYNLT1 (cow): T94‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays DYNC1I1 (mouse) Disrupts co-immunoprecipitation, in vitro ARHGEF2 (mouse) Induces activity, inhibited co-immunoprecipitation, in vitro