Curated Information
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Home > Curated Information Page > PubMed Id: 8119945
Strausfeld U, et al. (1994) Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis. J Biol Chem 269, 5989-6000 8119945
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T48-p - CDC25C (human)
Modsite: VCPDVPRtPVGkFLG SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): T48‑p, CDC25C iso3 (human): T48‑p, CDC25C (mouse): S48‑p, CDC25C (frog): T48‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T67-p - CDC25C (human)
Modsite: LsILsGGtPkRCLDL SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): T67‑p, CDC25C iso3 (human): , CDC25C (mouse): T66‑p, CDC25C (frog): T67‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S122-p - CDC25C (human)
Modsite: DQHLMKCsPAQLLCS SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S122‑p, CDC25C iso3 (human): , CDC25C (mouse): I121‑p, CDC25C (frog): L131‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T130-p - CDC25C (human)
Modsite: PAQLLCStPNGLDRG SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): T130‑p, CDC25C iso3 (human): T87‑p, CDC25C (mouse): T129‑p, CDC25C (frog): T138‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S168-p - CDC25C (human)
Modsite: SEMKyLGsPIttVPK SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S168‑p, CDC25C iso3 (human): S125‑p, CDC25C (mouse): S192‑p, CDC25C (frog): S205‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S214-p - CDC25C (human)
Modsite: sRsGLyRsPsMPENL SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S214‑p, CDC25C iso3 (human): S171‑p, CDC25C (mouse): , CDC25C (frog): S285‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  REF52 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)