Strausfeld U, et al. (1994) Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis. J Biol Chem 269, 5989-6000 8119945

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T48-p - CDC25C (human) ▲

Modsite: VCPDVPRtPVGkFLG SwissProt Entrez-Gene Orthologous residues CDC25C (human): T48‑p, CDC25C iso3 (human): T48‑p, CDC25C (mouse): S48‑p, CDC25C (frog): T48‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

T67-p - CDC25C (human) ▲

Modsite: LsILsGGtPkRCLDL SwissProt Entrez-Gene Orthologous residues CDC25C (human): T67‑p, CDC25C iso3 (human): ‑, CDC25C (mouse): T66‑p, CDC25C (frog): T67‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

S122-p - CDC25C (human) ▲

Modsite: DQHLMKCsPAQLLCS SwissProt Entrez-Gene Orthologous residues CDC25C (human): S122‑p, CDC25C iso3 (human): ‑, CDC25C (mouse): I121‑p, CDC25C (frog): L131‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

T130-p - CDC25C (human) ▲

Modsite: PAQLLCStPNGLDRG SwissProt Entrez-Gene Orthologous residues CDC25C (human): T130‑p, CDC25C iso3 (human): T87‑p, CDC25C (mouse): T129‑p, CDC25C (frog): T138‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

S168-p - CDC25C (human) ▲

Modsite: SEMKyLGsPIttVPK SwissProt Entrez-Gene Orthologous residues CDC25C (human): S168‑p, CDC25C iso3 (human): S125‑p, CDC25C (mouse): S192‑p, CDC25C (frog): S205‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

S214-p - CDC25C (human) ▲

Modsite: sRsGLyRsPsMPENL SwissProt Entrez-Gene Orthologous residues CDC25C (human): S214‑p, CDC25C iso3 (human): S171‑p, CDC25C (mouse): ‑, CDC25C (frog): S285‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Relevant cell lines - cell types - tissues:  REF52 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)