Man JH, et al. (2006) PIAS3 induction of PRB sumoylation represses PRB transactivation by destabilizing its retention in the nucleus. Nucleic Acids Res 34, 5552-66 17020914

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K7-sm - PR (human) ▲

Modsite: _MTELKAkGPRAPHV SwissProt Entrez-Gene Orthologous residues PR (human): K7‑sm, PR iso2 (human): ‑, PR (mouse): K7‑sm, PR (rat): K7‑sm, PR (chicken): K7‑sm Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), T47D (breast cell) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes SUMO LIGASE PIAS3 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, two-hybrid system, microscopy-colocalization, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes progesterone increase PIAS4 (human) increase marginal increase siRNA decrease PIAS3 siRNA Downstream Regulation Effect of modification (function):  intracellular localization, protein conformation, protein stabilization, sumoylation Effect of modification (process):  transcription, inhibited Comments:  induces sumoylation at other sites

K388-sm - PR (human) ▲

Modsite: QPPALKIkEEEEGAE SwissProt Entrez-Gene Orthologous residues PR (human): K388‑sm, PR iso2 (human): K224‑sm, PR (mouse): K388‑sm, PR (rat): K387‑sm, PR (chicken): K294‑sm Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), T47D (breast cell) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes SUMO LIGASE PIAS3 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, two-hybrid system, microscopy-colocalization, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes progesterone increase PIAS4 (human) increase marginal increase siRNA decrease PIAS3 siRNA Downstream Regulation Effect of modification (function):  intracellular localization, protein conformation, protein stabilization, sumoylation Effect of modification (process):  transcription, inhibited Comments:  induces sumoylation at other sites

K531-sm - PR (human) ▲

Modsite: GYQAAVLkEGLPQVY SwissProt Entrez-Gene Orthologous residues PR (human): K531‑sm, PR iso2 (human): K367‑sm, PR (mouse): K524‑sm, PR (rat): K521‑sm, PR (chicken): K385‑sm Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), HeLa (cervical), T47D (breast cell) Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes SUMO LIGASE PIAS3 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, two-hybrid system, microscopy-colocalization, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes progesterone increase PIAS4 (human) increase marginal increase siRNA decrease PIAS3 siRNA Downstream Regulation Effect of modification (function):  intracellular localization, protein conformation, protein stabilization, sumoylation Effect of modification (process):  transcription, inhibited Comments:  induces sumoylation at other sites