Deng X, et al. (2006) Bcl2's flexible loop domain regulates p53 binding and survival. Mol Cell Biol 26, 4421-34 16738310

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

T69-p - Bcl-2 (human) ▲

Modsite: SRDPVARtsPLQtPA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): T69‑p, Bcl‑2 (mouse): T69‑p, Bcl‑2 (rat): T69‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NSF/N1.H7 (myeloid) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cisplatin decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  apoptosis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays p53 (mouse) Disrupts co-immunoprecipitation, sequence-specific competitor BAX (mouse) Induces co-immunoprecipitation

S70-p - Bcl-2 (human) ▲

Modsite: RDPVARtsPLQtPAA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S70‑p, Bcl‑2 (mouse): S70‑p, Bcl‑2 (rat): S70‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NSF/N1.H7 (myeloid) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cisplatin decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  apoptosis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays p53 (mouse) Disrupts co-immunoprecipitation, sequence-specific competitor BAX (mouse) Induces co-immunoprecipitation

S87-p - Bcl-2 (human) ▲

Modsite: AAAGPALsPVPPVVH SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S87‑p, Bcl‑2 (mouse): S84‑p, Bcl‑2 (rat): S84‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NSF/N1.H7 (myeloid) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cisplatin decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  apoptosis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays BAX (mouse) Induces co-immunoprecipitation p53 (mouse) Disrupts co-immunoprecipitation, sequence-specific competitor