Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.7
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 16803855
Sakamoto K, et al. (2006) Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle. Am J Physiol Endocrinol Metab 291, E1031-7 16803855
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
muscle contraction Akt2 (mouse) inhibit treatment-induced increase knockout
Akt2 (mouse) no change compared to control knockout

T183-p - AMPKA1 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
muscle contraction Akt2 (mouse) no effect upon treatment-induced increase knockout
Akt2 (mouse) no change compared to control knockout

S21-p - GSK3A (mouse)
Modsite: sGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
muscle contraction Akt2 (mouse) inhibit treatment-induced increase knockout
Akt2 (mouse) decrease knockout

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
muscle contraction Akt2 (mouse) no effect upon treatment-induced increase knockout
Akt2 (mouse) no change compared to control knockout

S645-p - GYS1 (mouse)
Modsite: RPAsVPPsPsLsRHs SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S645‑p, GYS1 iso2 (human): S581‑p, GYS1 (mouse): S645‑p, GYS1 (rat): S645‑p, GYS1 (rabbit): S645‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction decrease
muscle contraction Akt2 (mouse) no effect upon treatment-induced decrease knockout
Akt2 (mouse) no change compared to control knockout

S649-p - GYS1 (mouse)
Modsite: VPPsPsLsRHssPHQ SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S649‑p, GYS1 iso2 (human): S585‑p, GYS1 (mouse): S649‑p, GYS1 (rat): S649‑p, GYS1 (rabbit): S649‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction decrease
muscle contraction Akt2 (mouse) no effect upon treatment-induced decrease knockout
Akt2 (mouse) no change compared to control knockout

S653-p - GYS1 (mouse)
Modsite: PsLsRHssPHQsEDE SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S653‑p, GYS1 iso2 (human): S589‑p, GYS1 (mouse): S653‑p, GYS1 (rat): S653‑p, GYS1 (rabbit): S653‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction decrease
muscle contraction Akt2 (mouse) no effect upon treatment-induced decrease knockout
Akt2 (mouse) no change compared to control knockout

S657-p - GYS1 (mouse)
Modsite: RHssPHQsEDEEEPR SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S657‑p, GYS1 iso2 (human): S593‑p, GYS1 (mouse): S657‑p, GYS1 (rat): S657‑p, GYS1 (rabbit): S657‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction decrease
muscle contraction Akt2 (mouse) no effect upon treatment-induced decrease knockout
Akt2 (mouse) no change compared to control knockout