Curated Information
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Home > Curated Information Page > PubMed Id: 12709442
Roos-Mattjus P, et al. (2003) Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling. J Biol Chem 278, 24428-37 12709442
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S272-p - RAD9A (human)
Modsite: LSDtDSHsQDLGsPE SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S272‑p, RAD9A (mouse): S272‑p, RAD9A (rat): S272‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, peptide sequencing, phospho-antibody
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
hydroxyurea increase
ionizing_radiation increase

S277-p - RAD9A (human)
Modsite: SHsQDLGsPERHQPV SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S277‑p, RAD9A (mouse): S277‑p, RAD9A (rat): S277‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S328-p - RAD9A (human)
Modsite: VLPsIsLsPGPQPPK SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S328‑p, RAD9A (mouse): P328‑p, RAD9A (rat): P328‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S336-p - RAD9A (human)
Modsite: PGPQPPKsPGPHsEE SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S336‑p, RAD9A (mouse): D336‑p, RAD9A (rat): D336‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S341-p - RAD9A (human)
Modsite: PKsPGPHsEEEDEAE SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S341‑p, RAD9A (mouse): S341‑p, RAD9A (rat): S341‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

T355-p - RAD9A (human)
Modsite: EPstVPGtPPPKKFR SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): T355‑p, RAD9A (mouse): T353‑p, RAD9A (rat): T356‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S375-p - RAD9A (human)
Modsite: SILAPVRsPQGPsPV SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S375‑p, RAD9A (mouse): S373‑p, RAD9A (rat): S376‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S380-p - RAD9A (human)
Modsite: VRsPQGPsPVLAEDs SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S380‑p, RAD9A (mouse): N378‑p, RAD9A (rat): N381‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse

S387-p - RAD9A (human)
Modsite: sPVLAEDsEGEG___ SwissProt Entrez-Gene
Orthologous residues
RAD9A (human): S387‑p, RAD9A (mouse): S385‑p, RAD9A (rat): S388‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  'stem, embryonic', K562 (erythroid)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse