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Home > Curated Information Page > PubMed Id: 28888989
Alcántara-Hernández R, et al. (2017) Noradrenaline, oxymetazoline and phorbol myristate acetate induce distinct functional actions and phosphorylation patterns of α1A-adrenergic receptors. Biochim Biophys Acta 1864, 2378-2388 28888989
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S220-p - ADRA1A (human)
Modsite: RESRGLKsGLKTDKs SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S220‑p, ADRA1A iso2 (human): S220‑p, ADRA1A iso8 (human): S220‑p, ADRA1A (mouse): S220‑p, ADRA1A (rat): S220‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase

S227-p - ADRA1A (human)
Modsite: sGLKTDKsDsEQVtL SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S227‑p, ADRA1A iso2 (human): S227‑p, ADRA1A iso8 (human): S227‑p, ADRA1A (mouse): S227‑p, ADRA1A (rat): S227‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
oxymetazoline increase

S229-p - ADRA1A (human)
Modsite: LKTDKsDsEQVtLRI SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S229‑p, ADRA1A iso2 (human): S229‑p, ADRA1A iso8 (human): S229‑p, ADRA1A (mouse): S229‑p, ADRA1A (rat): S229‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase

T233-p - ADRA1A (human)
Modsite: KsDsEQVtLRIHRkN SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): T233‑p, ADRA1A iso2 (human): T233‑p, ADRA1A iso8 (human): T233‑p, ADRA1A (mouse): T233‑p, ADRA1A (rat): T233‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine no change compared to control
oxymetazoline no change compared to control
phorbol_ester no change compared to control

S246-p - ADRA1A (human)
Modsite: kNAPAGGsGMAsAkT SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S246‑p, ADRA1A iso2 (human): S246‑p, ADRA1A iso8 (human): S246‑p, ADRA1A (mouse): S246‑p, ADRA1A (rat): G246‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
oxymetazoline increase
phorbol_ester increase

S250-p - ADRA1A (human)
Modsite: AGGsGMAsAkTkTHF SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S250‑p, ADRA1A iso2 (human): S250‑p, ADRA1A iso8 (human): S250‑p, ADRA1A (mouse): S250‑p, ADRA1A (rat): S250‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
phorbol_ester increase

S258-p - ADRA1A (human)
Modsite: AkTkTHFsVRLLKFS SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S258‑p, ADRA1A iso2 (human): S258‑p, ADRA1A iso8 (human): S258‑p, ADRA1A (mouse): S258‑p, ADRA1A (rat): S258‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S351-p - ADRA1A (human)
Modsite: QCLCRKQssKHALGY SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S351‑p, ADRA1A iso2 (human): S351‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S351‑p, ADRA1A (rat): S351‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S352-p - ADRA1A (human)
Modsite: CLCRKQssKHALGYT SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S352‑p, ADRA1A iso2 (human): S352‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S352‑p, ADRA1A (rat): S352‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S381-p - ADRA1A (human)
Modsite: MVRIPVGsREtFYRI SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S381‑p, ADRA1A iso2 (human): S381‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S381‑p, ADRA1A (rat): S381‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
oxymetazoline increase

T384-p - ADRA1A (human)
Modsite: IPVGsREtFYRIsKT SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): T384‑p, ADRA1A iso2 (human): T384‑p, ADRA1A iso8 (human): , ADRA1A (mouse): T384‑p, ADRA1A (rat): T384‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
oxymetazoline increase

S389-p - ADRA1A (human)
Modsite: REtFYRIsKTDGVCE SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S389‑p, ADRA1A iso2 (human): S389‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S389‑p, ADRA1A (rat): S389‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
norepinephrine increase
oxymetazoline increase

S401-p - ADRA1A (human)
Modsite: VCEWKFFssMPRGsA SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S401‑p, ADRA1A iso2 (human): S401‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S401‑p, ADRA1A (rat): S401‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S402-p - ADRA1A (human)
Modsite: CEWKFFssMPRGsAR SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S402‑p, ADRA1A iso2 (human): S402‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S402‑p, ADRA1A (rat): S402‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S407-p - ADRA1A (human)
Modsite: FssMPRGsARItVsK SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S407‑p, ADRA1A iso2 (human): S407‑p, ADRA1A iso8 (human): , ADRA1A (mouse): S407‑p, ADRA1A (rat): S407‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

T411-p - ADRA1A (human)
Modsite: PRGsARItVsKDQSS SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): T411‑p, ADRA1A iso2 (human): T411‑p, ADRA1A iso8 (human): , ADRA1A (mouse): T411‑p, ADRA1A (rat): T411‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

S413-p - ADRA1A (human)
Modsite: GsARItVsKDQSSCT SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): S413‑p, ADRA1A iso2 (human): S413‑p, ADRA1A iso8 (human): , ADRA1A (mouse): P413‑p, ADRA1A (rat): P413‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

T451-p - ADRA1A (human)
Modsite: DKNHQVPtIKVHTIS SwissProt Entrez-Gene
Orthologous residues
ADRA1A (human): T451‑p, ADRA1A iso2 (human): , ADRA1A iso8 (human): , ADRA1A (mouse): T451‑p, ADRA1A (rat): T451‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  LNCaP (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase