Curated Information
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Home > Curated Information Page > PubMed Id: 22375000
Moul├ędous L, et al. (2012) GRK2 protein-mediated transphosphorylation contributes to loss of function of ╬╝-opioid receptors induced by neuropeptide FF (NPFF2) receptors. J Biol Chem 287, 12736-49 22375000
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T372-p - MOR-1 (human)
Modsite: STRIRQNtRDHPSTA SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T372‑p, MOR‑1 iso5 (human): T372‑p, MOR‑1 (mouse): T370‑p, MOR‑1 (rat): T370‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
1DMe increase

S377-p - MOR-1 (human)
Modsite: QNTRDHPsTANTVDR SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): S377‑p, MOR‑1 iso5 (human): S377‑p, MOR‑1 (mouse): S375‑p, MOR‑1 (rat): S375‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK2 (human) siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
1DMe increase
DAMGO increase
clonidine no change compared to control
DAMGO, 1DMe increase
1DMe NPFFR2 (human) augment treatment-induced increase
naloxone 1DMe no effect upon treatment-induced increase
siRNA 1DMe inhibit treatment-induced increase GRK2 siRNA
PTX DAMGO augment treatment-induced increase
PTX 1DMe inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  receptor desensitization, induced

T378-p - MOR-1 (human)
Modsite: NTRDHPStANTVDRT SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T378‑p, MOR‑1 iso5 (human): T378‑p, MOR‑1 (mouse): T376‑p, MOR‑1 (rat): T376‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
1DMe increase