Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22349701
Sala-Vald├ęs M, et al. (2012) Association of syntenin-1 with M-RIP polarizes Rac-1 activation during chemotaxis and immune interactions. J Cell Sci 125, 1235-46 22349701
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

Y4-p - syntenin (human)
Modsite: ____MsLyPsLEDLk SwissProt Entrez-Gene
Orthologous residues
syntenin (human): Y4‑p, syntenin iso2 (human): Y4‑p, syntenin (mouse): Y4‑p, syntenin (rat): Y4‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  J774 (macrophage), lymphoblastoid
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CXCL12 increase
PP2 CXCL12 inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell motility, induced, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MPRIP (human) Induces microscopy-colocalization, co-immunoprecipitation
Comments:  T-cell chemotaxis induced, Rac1 activation and translocation to plasma membrane induced

Y46-p - syntenin (human)
Modsite: IPHDGNLyPRLyPEL SwissProt Entrez-Gene
Orthologous residues
syntenin (human): Y46‑p, syntenin iso2 (human): Y46‑p, syntenin (mouse): Y47‑p, syntenin (rat): Y48‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  lymphoblastoid
Cellular systems studied:  primary cultured cells
Species studied:  human

Y50-p - syntenin (human)
Modsite: GNLyPRLyPELsQyM SwissProt Entrez-Gene
Orthologous residues
syntenin (human): Y50‑p, syntenin iso2 (human): Y50‑p, syntenin (mouse): Y51‑p, syntenin (rat): Y52‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  lymphoblastoid
Cellular systems studied:  primary cultured cells
Species studied:  human

Y56-p - syntenin (human)
Modsite: LyPELsQyMGLsLNE SwissProt Entrez-Gene
Orthologous residues
syntenin (human): Y56‑p, syntenin iso2 (human): Y56‑p, syntenin (mouse): Y57‑p, syntenin (rat): Y58‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  lymphoblastoid
Cellular systems studied:  primary cultured cells
Species studied:  human