Wang J, Wang Y, Lu L (2012) De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells. J Biol Chem 287, 12469-79 22354964

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K74-sm - CTCF (human) ▲

Modsite: DPTLLQMkTEVMEGT SwissProt Entrez-Gene Orthologous residues CTCF (human): K74‑sm, CTCF (mouse): K74‑sm, CTCF (rat): K74‑sm, CTCF (chicken): K74‑sm Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), alpha-TC6 (pancreatic), hTCEpi (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DESUMOYLASE SENP1 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme not involved in hypoxic stress-induced de-SUMOylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 decrease hypoxia decrease heat_shock no change compared to control UV no change compared to control hyperosmotic shock no change compared to control SENP3 (human) no change compared to control SENP5 (human) no change compared to control siRNA increase SENP1 siRNA Downstream Regulation Effect of modification (process):  transcription, induced

K689-sm - CTCF (human) ▲

Modsite: ENIIVEVkKEPDAEP SwissProt Entrez-Gene Orthologous residues CTCF (human): K689‑sm, CTCF (mouse): K698‑sm, CTCF (rat): K699‑sm, CTCF (chicken): K691‑sm Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), alpha-TC6 (pancreatic), hTCEpi (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DESUMOYLASE SENP1 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme not involved in hypoxic stress-induced de-SUMOylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 decrease hypoxia decrease heat_shock no change compared to control UV no change compared to control hyperosmotic shock no change compared to control SENP3 (human) no change compared to control SENP5 (human) no change compared to control siRNA increase SENP1 siRNA Downstream Regulation Effect of modification (process):  transcription, induced