Curated Information
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Home > Curated Information Page > PubMed Id: 10102632
Dougher M, Terman BI (1999) Autophosphorylation of KDR in the kinase domain is required for maximal VEGF-stimulated kinase activity and receptor internalization. Oncogene 18, 1619-27 10102632
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y951-p - VEGFR2 (human)
Modsite: RFRQGKDyVGAIPVD SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y951‑p, VEGFR2 (mouse): Y949‑p, VEGFR2 (rat): Y947‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE VEGFR2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE VEGFR2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, receptor internalization, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces phosphorylation electrophoretic visualization, co-immunoprecipitation

Y996-p - VEGFR2 (human)
Modsite: EEAPEDLyKDFLTLE SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y996‑p, VEGFR2 (mouse): Y994‑p, VEGFR2 (rat): Y992‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE VEGFR2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE VEGFR2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, receptor internalization, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces phosphorylation electrophoretic visualization, co-immunoprecipitation

Y1054-p - VEGFR2 (human)
Modsite: FGLARDIykDPDyVR SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y1054‑p, VEGFR2 (mouse): Y1052‑p, VEGFR2 (rat): Y1050‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE VEGFR2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE VEGFR2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, receptor internalization, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces phosphorylation electrophoretic visualization, co-immunoprecipitation

Y1059-p - VEGFR2 (human)
Modsite: DIykDPDyVRKGDAR SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y1059‑p, VEGFR2 (mouse): Y1057‑p, VEGFR2 (rat): Y1055‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE VEGFR2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE VEGFR2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, receptor internalization, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces phosphorylation electrophoretic visualization, co-immunoprecipitation