Curated Information
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Home > Curated Information Page > PubMed Id: 21962514
Holzer RG, et al. (2011) Saturated fatty acids induce c-Src clustering within membrane subdomains, leading to JNK activation. Cell 147, 173-84 21962514
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S473-p - Akt1 (mouse)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate decrease
palmitate insulin inhibit treatment-induced increase
insulin increase Src, Yes, Fyn, (SYF) cells
palmitate decrease SYF cells
palmitate insulin no effect upon treatment-induced increase
insulin increase SYF + Src
palmitate decrease SYF + Src cells
palmitate insulin inhibit treatment-induced increase

T183-p - JNK1 (mouse)
Modsite: AGTSFMMtPYVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells
palmitate Src (mouse) increase SYF + Src cells

Y185-p - JNK1 (mouse)
Modsite: TSFMMTPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells
palmitate Src (mouse) increase SYF + Src cells

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPYVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells

Y185-p - JNK2 (mouse)
Modsite: TNFMMTPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells

T278-p - MLK3 (mouse)
Modsite: LAREWHKtTQMSAAG SwissProt Entrez-Gene
Orthologous residues
MLK3 (human): T277‑p, MLK3 (mouse): T278‑p, MLK3 (rat): T278‑p, MLK3 (cow): T277‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells

S282-p - MLK3 (mouse)
Modsite: WHKTTQMsAAGTYAW SwissProt Entrez-Gene
Orthologous residues
MLK3 (human): S281‑p, MLK3 (mouse): S282‑p, MLK3 (rat): S282‑p, MLK3 (cow): S281‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate Src (mouse) increase SYF + Src cells

Y424-p - Src (mouse)
Modsite: RLIEDNEyTARQGAK SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate increase Src membrane redistribution
saturated_fatty_acid increase
palmitoleic_acid palmitate inhibit treatment-induced increase inhibit Src membrane redistribution
polyunsaturated_fatty_acid palmitate inhibit treatment-induced increase inhibits Src membrane redistribution
high-fat diet increase Src segregation and activation

Y527-p - Src (mouse)
Modsite: LQAFLEDyFTSTEPQ SwissProt Entrez-Gene
Orthologous residues
Src (human): Y522‑p, Src iso2 (human): Y528‑p, Src (mouse): Y527‑p, Src iso2 (mouse): Y521‑p, Src (rat): Y522‑p, Src (chicken): Y519‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse