Waraky A, et al. (2017) Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem 292, 18227-18239 28924044

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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Y60-p - PCNA (human) ▲

Modsite: RSEGFDTyrCDRNLA SwissProt Entrez-Gene Orthologous residues PCNA (human): Y60‑p, PCNA (mouse): Y60‑p, PCNA (rat): Y60‑p, PCNA (chicken): Y60‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), stem Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IGF1R (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IGF1R (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  ubiquitination Effect of modification (process):  cell cycle regulation

Y133-p - PCNA (human) ▲

Modsite: LGIPEQEysCVVkMP SwissProt Entrez-Gene Orthologous residues PCNA (human): Y133‑p, PCNA (mouse): Y133‑p, PCNA (rat): Y133‑p, PCNA (chicken): Y133‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), stem Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IGF1R (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IGF1R (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  ubiquitination Effect of modification (process):  cell cycle regulation

Y250-p - PCNA (human) ▲

Modsite: DMGHLkYyLAPkIED SwissProt Entrez-Gene Orthologous residues PCNA (human): Y250‑p, PCNA (mouse): Y250‑p, PCNA (rat): Y250‑p, PCNA (chicken): Y250‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), stem Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IGF1R (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IGF1R (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  ubiquitination Effect of modification (process):  cell cycle regulation