Curated Information
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Home > Curated Information Page > PubMed Id: 28877472
Zimmermann S, et al. (2017) ALPK1- and TIFA-Dependent Innate Immune Response Triggered by the Helicobacter pylori Type IV Secretion System. Cell Rep 20, 2384-2395 28877472
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S32-p - IkB-alpha (human)
Modsite: LLDDRHDsGLDsMkD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  gastric cancer, gastric carcinoma
Relevant cell lines - cell types - tissues:  AGS (gastric)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
AlphaK3 (human) increase AlphaK3 -/- decrease
TIFA (human) increase TIFA -/- decrease

S536-p - NFkB-p65 (human)
Modsite: sGDEDFSsIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  gastric cancer, gastric carcinoma
Relevant cell lines - cell types - tissues:  AGS (gastric)
Cellular systems studied:  cell lines
Species studied:  human

T184-p - TAK1 (human)
Modsite: GtACDIQtHMtNNKG SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T184‑p, TAK1 (mouse): T184‑p, TAK1 iso3 (mouse): T184‑p, TAK1 (rat): T184‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  gastric cancer, gastric carcinoma
Relevant cell lines - cell types - tissues:  AGS (gastric)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
AlphaK3 (human) increase AlphaK3 -/- decrease
TIFA (human) increase TIFA -/- decrease

T187-p - TAK1 (human)
Modsite: CDIQtHMtNNKGsAA SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T187‑p, TAK1 (mouse): T187‑p, TAK1 iso3 (mouse): T187‑p, TAK1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  gastric cancer, gastric carcinoma
Relevant cell lines - cell types - tissues:  AGS (gastric)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
AlphaK3 (human) increase AlphaK3 -/- decrease
TIFA (human) increase TIFA -/- decrease

T9-p - TIFA (human)
Modsite: TSFEDADtEETVTCL SwissProt Entrez-Gene
Orthologous residues
TIFA (human): T9‑p, TIFA (mouse): T9‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  gastric cancer, gastric carcinoma
Relevant cell lines - cell types - tissues:  AGS (gastric)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Comments:  p65 translocation and TIFAsome formation