Curated Information
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Home > Curated Information Page > PubMed Id: 28892081
Lee JH, et al. (2017) A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1. Nat Cell Biol 19, 1260-1273 28892081
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S82-p - Snail1 (human)
Modsite: ASLRLQEsPRVAELT SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S82‑p, Snail1 (mouse): S82‑p

S104-p - Snail1 (human)
Modsite: GKGsQPPsPPsPAPs SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S104‑p, Snail1 (mouse): S104‑p

K206-ub - Snail1 (human)
Modsite: VRtHtGEkPFSCPHC SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): K206‑ub, Snail1 (mouse): K206‑ub
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 4T1 (breast cell), MCF10CA1h (epithelial), MDA-MB-231 (breast cell), NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TNFAIP3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TNFAIP3 (human) co-immunoprecipitation, activation of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  metastasis

K234-ub - Snail1 (human)
Modsite: LQTHSDVkkYQCQAC SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): K234‑ub, Snail1 (mouse): K234‑ub
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 4T1 (breast cell), MCF10CA1h (epithelial), MDA-MB-231 (breast cell), NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TNFAIP3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TNFAIP3 (human) co-immunoprecipitation, activation of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  metastasis

K235-ub - Snail1 (human)
Modsite: QTHSDVkkYQCQACA SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): K235‑ub, Snail1 (mouse): R235‑ub
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 4T1 (breast cell), MCF10CA1h (epithelial), MDA-MB-231 (breast cell), NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TNFAIP3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TNFAIP3 (human) co-immunoprecipitation, activation of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  metastasis