Chiu YT, et al. (2017) Agonist-Dependent and -Independent κ Opioid Receptor Phosphorylation: Distinct Phosphorylation Patterns and Different Cellular Outcomes. Mol Pharmacol 92, 588-600 28893975

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S356-p - KOR-1 (mouse) ▲

Modsite: KMRMERQstNRVRNt SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): S356‑p, KOR‑1 (mouse): S356‑p, KOR‑1 (rat): S356‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK5 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of wild-type enzyme KINASE GRK6 (mouse) siRNA inhibition of enzyme, phospho-antibody KINASE GRK2 (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody KINASE GRK3 (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase PTX U50488 inhibit treatment-induced increase GF109203X decrease Go_6976 no change compared to control phorbol_ester increase Downstream Regulation Effect of modification (function):  receptor internalization, induced

T357-p - KOR-1 (mouse) ▲

Modsite: MRMERQstNRVRNtV SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): T357‑p, KOR‑1 (mouse): T357‑p, KOR‑1 (rat): T357‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK5 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of wild-type enzyme KINASE GRK2 (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody KINASE GRK6 (mouse) siRNA inhibition of enzyme, phospho-antibody KINASE GRK3 (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase PTX U50488 inhibit treatment-induced increase GF109203X decrease Go_6976 no change compared to control phorbol_ester increase Downstream Regulation Effect of modification (function):  receptor internalization, induced

T363-p - KOR-1 (mouse) ▲

Modsite: stNRVRNtVQDPAsM SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): T363‑p, KOR‑1 (mouse): T363‑p, KOR‑1 (rat): T363‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK6 (mouse) siRNA inhibition of enzyme, phospho-antibody KINASE GRK5 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase PTX U50488 inhibit treatment-induced increase GF109203X decrease Go_6976 no change compared to control phorbol_ester increase chelerythrine decrease

S369-p - KOR-1 (mouse) ▲

Modsite: NtVQDPAsMRDVGGM SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): Y369‑p, KOR‑1 (mouse): S369‑p, KOR‑1 (rat): S369‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GRK5 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of wild-type enzyme KINASE GRK6 (mouse) siRNA inhibition of enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase PTX U50488 inhibit treatment-induced increase GF109203X decrease Go_6976 no change compared to control phorbol_ester increase chelerythrine decrease